PUBLICATION
Comparative venomics reveals the complex prey capture strategy of the piscivorous cone snail Conus catus
- Authors
- Himaya, S.W., Jin, A.H., Dutertre, S., Giacomotto, J., Mohialdeen, H., Vetter, I., Alewood, P.F., Lewis, R.J.
- ID
- ZDB-PUB-150901-1
- Date
- 2015
- Source
- Journal of Proteome Research 14(10): 4372-81 (Journal)
- Registered Authors
- Giacomotto, Jean
- Keywords
- Conus catus, transcriptome, kA-conotoxin, glycosylation, predatory-evoked venom, intraspecific variation
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Aquatic Organisms
- Calcium Channel Blockers/chemistry
- Calcium Channel Blockers/isolation & purification*
- Calcium Channel Blockers/toxicity
- Calcium Channels/metabolism
- Conotoxins/chemistry
- Conotoxins/isolation & purification*
- Conotoxins/toxicity
- Conus Snail/chemistry*
- Conus Snail/physiology
- Molecular Sequence Annotation
- Molecular Sequence Data
- Mollusk Venoms/chemistry
- Mollusk Venoms/isolation & purification*
- Mollusk Venoms/toxicity
- Motor Activity/drug effects
- Nicotinic Antagonists/chemistry
- Nicotinic Antagonists/isolation & purification*
- Nicotinic Antagonists/toxicity
- Potassium Channel Blockers/chemistry
- Potassium Channel Blockers/isolation & purification*
- Potassium Channel Blockers/toxicity
- Potassium Channels/metabolism
- Predatory Behavior/physiology
- Receptors, Nicotinic/metabolism
- Species Specificity
- Transcriptome
- Zebrafish/physiology
- PubMed
- 26322961 Full text @ J. Proteome Res.
Citation
Himaya, S.W., Jin, A.H., Dutertre, S., Giacomotto, J., Mohialdeen, H., Vetter, I., Alewood, P.F., Lewis, R.J. (2015) Comparative venomics reveals the complex prey capture strategy of the piscivorous cone snail Conus catus. Journal of Proteome Research. 14(10):4372-81.
Abstract
Venomous marine cone snails produce a unique and remarkably diverse range of venom peptides (conotoxins and conopeptides) that have proven invaluable as pharmacological probes and leads to new therapies. Conus catus is 'hook-and-line' fish hunter from clade I, with ~20 conotoxins identified, including the analgesic ω-conotoxin CVID (AM336). The current study unravels the venom composition of C. catus with tandem mass spectrometry and 454 sequencing data. From the venom gland transcriptome, 104 precursors were recovered from 11 superfamilies, with superfamily A (especially κA-) conotoxins dominating (77%) their venom. Proteomic analysis confirmed the κA-conotoxins dominated the predation-evoked milked venom of each of six C. catus analysed and revealed remarkable intraspecific variation in both the intensity and type of conotoxins. High-throughput FLIPR assays revealed that the predation-evoked venom contained a range of conotoxins targeting the nAChR, Cav and Nav ion channels, consistent with α- and ω-conotoxins being used for predation by C. catus. However, the κA-conotoxins did not act at these targets but induced potent and rapid immobilisation followed by bursts of activity and finally paralysis when injected intramuscularly in zebrafish. Our venomics approach revealed the complexity of the envenomation strategy used by C. catus, which contains a mix of both excitatory and inhibitory venom peptides.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping