PUBLICATION
Cryptotanshinone, a novel tumor angiogenesis inhibitor, destabilizes tumor necrosis factor-α mRNA via decreasing nuclear-cytoplasmic translocation of RNA-binding protein HuR
- Authors
- Zhu, Z., Zhao, Y., Li, J., Tao, L., Shi, P., Wei, Z., Sheng, X., Shen, D., Liu, Z., Zhou, L., Tian, C., Fan, F., Shen, C., Zhu, P., Wang, A., Chen, W., Zhao, Q., Lu, Y.
- ID
- ZDB-PUB-150828-5
- Date
- 2016
- Source
- Molecular Carcinogenesis 55(10): 1399-410 (Journal)
- Registered Authors
- Zhao, Qingshun
- Keywords
- HuR, angiogenesis, cryptotanshinone, mRNA stability, tumor necrosis factor-α
- MeSH Terms
-
- ELAV-Like Protein 1/metabolism*
- Humans
- Signal Transduction/drug effects
- Cell Line, Tumor
- Gene Expression Regulation, Neoplastic/drug effects
- Cytoplasm/drug effects
- Cytoplasm/metabolism
- Cell Nucleus/drug effects
- Cell Nucleus/metabolism
- Cell Movement
- Xenograft Model Antitumor Assays
- Animals
- Angiogenesis Inhibitors/administration & dosage*
- Angiogenesis Inhibitors/pharmacology
- Cell Proliferation/drug effects
- Zebrafish
- Neoplasms/drug therapy*
- Mice
- RNA, Messenger/drug effects
- RNA, Messenger/metabolism
- Phenanthrenes/administration & dosage*
- Phenanthrenes/pharmacology
- RNA Stability/drug effects
- Human Umbilical Vein Endothelial Cells
- Tumor Necrosis Factor-alpha/genetics*
- PubMed
- 26310813 Full text @ Mol. Carcinog.
Citation
Zhu, Z., Zhao, Y., Li, J., Tao, L., Shi, P., Wei, Z., Sheng, X., Shen, D., Liu, Z., Zhou, L., Tian, C., Fan, F., Shen, C., Zhu, P., Wang, A., Chen, W., Zhao, Q., Lu, Y. (2016) Cryptotanshinone, a novel tumor angiogenesis inhibitor, destabilizes tumor necrosis factor-α mRNA via decreasing nuclear-cytoplasmic translocation of RNA-binding protein HuR. Molecular Carcinogenesis. 55(10):1399-410.
Abstract
Cryptotanshinone (CT), one major lipophilic component isolated from Salvia miltiorrhiza Bunge, has shown to possess chemopreventive properties against various types of cancer cells. In this study, CT was shown to be a potent anti-angiogenic agent in zebrafish, and mouse models and could limit tumor growth by inhibiting tumor angiogenesis. We further found that CT could inhibit the proliferation, migration, angiogenic sprouting, and tube formation of HUVECs. In addition, we demonstrated that CT could lower the level of TNF-α due to the destabilization of TNF-α mRNA, which associated with regulating 3'-untranslated region (3'-UTR) of TNF-α and preventing the translocation of RNA binding protein, HuR, from the nucleus to the cytoplasm. Moreover, the underlying mechanism responsible for the regulation in angiogenesis by CT was partially related to the suppression of NF-κB, and STAT3 activity. Based on the abilities of CT in targeting tumor cells, inhibiting angiogenesis, and destroying tumor vasculature, CT is worthy of further investigation for preventive, and therapeutic purposes in cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping