PUBLICATION
The secreted fructose 1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture
- Authors
- Sun, Z., Shen, B., Wu, H., Zhou, X., Wang, Q., Xiao, J., Zhang, Y.
- ID
- ZDB-PUB-150811-7
- Date
- 2015
- Source
- Fish & shellfish immunology 46(2): 638-47 (Journal)
- Registered Authors
- Keywords
- FBA, aquatic pathogenic bacteria, broad spectrum vaccine, cross-protection, locations
- MeSH Terms
-
- Adaptive Immunity
- Animals
- Aquaculture
- Bacteria/immunology*
- Bacterial Proteins/genetics
- Bacterial Proteins/immunology*
- Bacterial Vaccines/genetics
- Bacterial Vaccines/immunology*
- Flatfishes/immunology*
- Fructose-Bisphosphate Aldolase/genetics
- Fructose-Bisphosphate Aldolase/immunology*
- Immunity, Innate
- Sequence Analysis, DNA/veterinary
- Zebrafish/immunology*
- PubMed
- 26256425 Full text @ Fish Shellfish Immunol.
Citation
Sun, Z., Shen, B., Wu, H., Zhou, X., Wang, Q., Xiao, J., Zhang, Y. (2015) The secreted fructose 1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture. Fish & shellfish immunology. 46(2):638-47.
Abstract
The development of aquaculture has been hampered by different aquatic pathogens that can cause edwardsiellosis, vibriosis, or other diseases. Therefore, developing a broad spectrum vaccine against different fish diseases is necessary. In this study, fructose 1,6-bisphosphate aldolase (FBA), a conserved enzyme in the glycolytic pathway, was demonstrated to be located in the non-cytoplasmic components of five aquatic pathogenic bacteria and exhibited remarkable protection and cross-protection against these pathogens in turbot and zebrafish. Further analysis revealed that sera sampled from vaccinated turbot had a high level of specific antibody and bactericidal activity against these pathogens. Meanwhile, the increased expressions of immune response-related genes associated with antigen recognition and presentation indicated that the adaptive immune response was effectively aroused. Taken together, our results suggest that FBA can be utilized as a broad-spectrum vaccine against various pathogenic bacteria of aquaculture in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping