PUBLICATION
The N-terminal acetyltransferase Naa10 is essential for zebrafish development
- Authors
- Ree, R.M., Myklebust, L.M., Thiel, P., Foyn, H., Fladmark, K.E., Arnesen, T.
- ID
- ZDB-PUB-150808-2
- Date
- 2015
- Source
- Bioscience Reports 35(5): (Journal)
- Registered Authors
- Keywords
- abnormal development, Danio rerio, Nα-acetyltransferase 10 (Naa10), N-terminal acetyltransferase A (NatA), N-terminal acetyltransferase (NAT), N-terminal acetylation
- MeSH Terms
-
- Acetylation
- Amino Acid Sequence
- Animals
- Gene Knockdown Techniques
- Humans
- Molecular Sequence Data
- N-Terminal Acetyltransferase A/chemistry
- N-Terminal Acetyltransferase A/genetics*
- N-Terminal Acetyltransferase E
- Phylogeny
- Sequence Alignment
- Substrate Specificity
- Zebrafish/abnormalities
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 26251455 Full text @ Biosci. Rep.
Citation
Ree, R.M., Myklebust, L.M., Thiel, P., Foyn, H., Fladmark, K.E., Arnesen, T. (2015) The N-terminal acetyltransferase Naa10 is essential for zebrafish development. Bioscience Reports. 35(5).
Abstract
N-terminal acetylation, catalyzed by N-terminal acetyltransferases (NATs), is among the most common protein modifications in eukaryotes and involves the transfer of an acetyl group from acetyl-CoA to the a-amino group of the first amino acid. Functions of N-terminal acetylation include protein degradation and subcellular targeting. Recent findings in humans indicate that a dysfunctional N-alpha acetyltransferase (Naa) 10, the catalytic subunit of NatA, the major NAT, is associated with lethality during infancy. Here, we identified the Danio rerio orthologue zNaa10. In vitro N-terminal acetylation assays revealed that zNaa10 has NAT-activity with substrate specificity highly similar to that of human Naa10. Spatiotemporal expression pattern was determined by in situ hybridization, showing ubiquitous expression with especially strong staining in brain and eye. By morpholino-mediated knockdown, we demonstrated that naa10 morphants displayed increased lethality, growth retardation and developmental abnormalities like bent axis, abnormal eyes and bent tails. In conclusion, we identified the zebrafish Naa10 orthologue, and revealed that it is essential for normal development and viability of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping