PUBLICATION
Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1
- Authors
- Mukhopadhyay, D., Priya, P., Chattopadhyay, A.
- ID
- ZDB-PUB-150808-14
- Date
- 2015
- Source
- Environmental Toxicology and Pharmacology 40: 352-359 (Journal)
- Registered Authors
- Keywords
- Brain, Neurotoxicity, Nrf2, Reactive oxygen species, Sodium fluoride, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Brain/metabolism
- Carrier Proteins/genetics
- Carrier Proteins/metabolism
- Gene Expression Regulation/drug effects
- Genetic Markers/genetics
- NF-E2-Related Factor 2/genetics
- NF-E2-Related Factor 2/metabolism
- RNA, Messenger/drug effects*
- RNA, Messenger/genetics
- Reactive Oxygen Species/metabolism
- Signal Transduction/drug effects
- Sodium Fluoride/toxicity*
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 26245810 Full text @ Environ. Toxicol. Pharmacol.
Citation
Mukhopadhyay, D., Priya, P., Chattopadhyay, A. (2015) Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1. Environmental Toxicology and Pharmacology. 40:352-359.
Abstract
Sodium fluoride (NaF), used as pesticides and for industrial purposes are deposited in the water bodies and therefore affects its biota. Zebrafish exposed to NaF in laboratory condition showed hyperactivity and frequent surfacing activity, somersaulting and vertical swimming pattern as compared to the control group. Reactive oxygen species level was elevated and glutathione level was depleted along with increased malondialdehyde content in the brain. Levels of glutathione-s-transferase (GST), catalase (CAT) and superoxide dismutase were also elevated in the treatment groups. Expression of mRNA of nuclear factor erythroid 2 related factor 2 (Nrf2) and its inhibitor Kelch-like ECH-associated protein 1 (Keap1) during stress condition were observed along with Gst, Cat, NADPH: quinone oxidoreductase 1(Nqo1) and p38. Except Keap1, all other genes exhibited elevated expression. Nrf2/Keap1 proteins had similar expression pattern as their corresponding mRNA. The findings in this study might help to understand the molecular mechanism of fluoride induced neurotoxicity in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping