PUBLICATION
Expansion of CRISPR/Cas9 genome targeting sites in zebrafish by Csy4-based RNA processing
- Authors
- Qin, W., Liang, F., Feng, Y., Bai, H., Yan, R., Li, S., Lin, S.
- ID
- ZDB-PUB-150805-9
- Date
- 2015
- Source
- Cell Research 25(9): 1074-7 (Journal)
- Registered Authors
- Qing, Wei
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Binding Sites
- CRISPR-Cas Systems/genetics*
- Genome*
- Monophenol Monooxygenase/genetics
- Monophenol Monooxygenase/metabolism
- RNA, Guide, Kinetoplastida/metabolism*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 26238401 Full text @ Cell Res.
Citation
Qin, W., Liang, F., Feng, Y., Bai, H., Yan, R., Li, S., Lin, S. (2015) Expansion of CRISPR/Cas9 genome targeting sites in zebrafish by Csy4-based RNA processing. Cell Research. 25(9):1074-7.
Abstract
With its advantages of simple design and cost-efficiency, the CRISPR/Cas9 technology has been widely adapted for genome editing in different species including zebrafish1. In zebrafish studies, guide RNA (gRNA) is usually produced via in vitro transcription followed by microinjection with Cas9 mRNA into embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping