PUBLICATION
Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio)
- Authors
- Stancová, V., Ziková, A., Svobodová, Z., Kloas, W.
- ID
- ZDB-PUB-150804-8
- Date
- 2015
- Source
- Environmental Toxicology and Pharmacology 40: 343-348 (Journal)
- Registered Authors
- Keywords
- Fish, Naproxen, Oxidative stress, mRNA expression
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents, Non-Steroidal/adverse effects*
- Antioxidants/metabolism
- Catalase/genetics*
- Gene Expression Regulation, Enzymologic/drug effects
- Glutathione Transferase/genetics*
- Intestines/enzymology*
- Ion Channels/genetics*
- Liver/enzymology
- Mitochondrial Proteins/genetics*
- Naproxen/adverse effects*
- Uncoupling Protein 2
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 26233559 Full text @ Environ. Toxicol. Pharmacol.
Citation
Stancová, V., Ziková, A., Svobodová, Z., Kloas, W. (2015) Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio). Environmental Toxicology and Pharmacology. 40:343-348.
Abstract
The aim of this study was to investigate the effects of naproxen on the gene expression of antioxidant enzymes in adult zebrafish. Surprisingly, after 2 weeks exposure no significant effect on the mRNA expression of the target genes was found in the liver. However, mRNA levels of three genes were altered significantly in the intestine. The expression of Ucp-2 decreased at the environmental concentration of 1μg/L while mRNA expression of GST p2 increased at the concentration of 100μg/L. The mRNA level for the antioxidant enzyme CAT was up-regulated significantly at both the concentrations used. Exposure to naproxen caused only moderate effects on the expression of antioxidant genes in the intestine rather than in the liver, which demonstrates that the intestine is more sensitive to waterborne naproxen exposure than the liver. Interestingly, the adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study that has focused on transcriptional effects of naproxen on zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping