Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish
- Hu, P., Liu, M., Zhang, D., Wang, J., Niu, H., Liu, Y., Wu, Z., Han, B., Zhai, W., Shen, Y., Chen, L.
- Nucleic acids research 43(19): 9198-213 (Journal)
- Registered Authors
- MeSH Terms
- Cell Line
- Cold-Shock Response/genetics*
- Gene Expression Profiling
- Gene Expression Regulation*
- Gene Ontology
- Gene Regulatory Networks*
- Nucleotide Motifs
- Organ Specificity
- Promoter Regions, Genetic*
- Protein Interaction Mapping
- Transcription Factors/metabolism
- 26227973 Full text @ Nucleic Acids Res.
Hu, P., Liu, M., Zhang, D., Wang, J., Niu, H., Liu, Y., Wu, Z., Han, B., Zhai, W., Shen, Y., Chen, L. (2015) Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish. Nucleic acids research. 43(19):9198-213.
The transcriptional programs of ectothermic teleosts are directly influenced by water temperature. However, the cis- and trans-factors governing cold responses are not well characterized. We profiled transcriptional changes in eight zebrafish tissues exposed to mildly and severely cold temperatures using RNA-Seq. A total of 1943 differentially expressed genes (DEGs) were identified, from which 34 clusters representing distinct tissue and temperature response expression patterns were derived using the k-means fuzzy clustering algorithm. The promoter regions of the clustered DEGs that demonstrated strong co-regulation were analysed for enriched cis-regulatory elements with a motif discovery program, DREME. Seventeen motifs, ten known and seven novel, were identified, which covered 23% of the DEGs. Two motifs predicted to be the binding sites for the transcription factors Bcl6 and Jun, respectively, were chosen for experimental verification, and they demonstrated the expected cold-induced and cold-repressed patterns of gene regulation. Protein interaction modeling of the network components followed by experimental validation suggested that Jun physically interacts with Bcl6 and might be a hub factor that orchestrates the cold response in zebrafish. Thus, the methodology used and the regulatory networks uncovered in this study provide a foundation for exploring the mechanisms of cold adaptation in teleosts.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes