|ZFIN ID: ZDB-PUB-150801-4|
Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish
Hu, P., Liu, M., Zhang, D., Wang, J., Niu, H., Liu, Y., Wu, Z., Han, B., Zhai, W., Shen, Y., Chen, L.
|Source:||Nucleic acids research 43(19): 9198-213 (Journal)|
|PubMed:||26227973 Full text @ Nucleic Acids Res.|
Hu, P., Liu, M., Zhang, D., Wang, J., Niu, H., Liu, Y., Wu, Z., Han, B., Zhai, W., Shen, Y., Chen, L. (2015) Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish. Nucleic acids research. 43(19):9198-213.
ABSTRACTThe transcriptional programs of ectothermic teleosts are directly influenced by water temperature. However, the cis- and trans-factors governing cold responses are not well characterized. We profiled transcriptional changes in eight zebrafish tissues exposed to mildly and severely cold temperatures using RNA-Seq. A total of 1943 differentially expressed genes (DEGs) were identified, from which 34 clusters representing distinct tissue and temperature response expression patterns were derived using the k-means fuzzy clustering algorithm. The promoter regions of the clustered DEGs that demonstrated strong co-regulation were analysed for enriched cis-regulatory elements with a motif discovery program, DREME. Seventeen motifs, ten known and seven novel, were identified, which covered 23% of the DEGs. Two motifs predicted to be the binding sites for the transcription factors Bcl6 and Jun, respectively, were chosen for experimental verification, and they demonstrated the expected cold-induced and cold-repressed patterns of gene regulation. Protein interaction modeling of the network components followed by experimental validation suggested that Jun physically interacts with Bcl6 and might be a hub factor that orchestrates the cold response in zebrafish. Thus, the methodology used and the regulatory networks uncovered in this study provide a foundation for exploring the mechanisms of cold adaptation in teleosts.