ZFIN ID: ZDB-PUB-150729-5
Myristoylation profiling in human cells and zebrafish
Broncel, M., Serwa, R.A., Ciepla, P., Krause, E., Dallman, M.J., Magee, A.I., Tate, E.W.
Date: 2015
Source: Data in brief   4: 379-83 (Journal)
Registered Authors: Dallman, Maggie
Keywords: Modification site, Myristoylation, Protein modification, Tagging by substrate
MeSH Terms: none
PubMed: 26217820 Full text @ Data Brief
Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (VizcaĆ­no et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.