PUBLICATION
Myristoylation profiling in human cells and zebrafish
- Authors
- Broncel, M., Serwa, R.A., Ciepla, P., Krause, E., Dallman, M.J., Magee, A.I., Tate, E.W.
- ID
- ZDB-PUB-150729-5
- Date
- 2015
- Source
- Data in brief 4: 379-83 (Journal)
- Registered Authors
- Dallman, Maggie
- Keywords
- Modification site, Myristoylation, Protein modification, Tagging by substrate
- MeSH Terms
- none
- PubMed
- 26217820 Full text @ Data Brief
Citation
Broncel, M., Serwa, R.A., Ciepla, P., Krause, E., Dallman, M.J., Magee, A.I., Tate, E.W. (2015) Myristoylation profiling in human cells and zebrafish. Data in brief. 4:379-83.
Abstract
Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping