PUBLICATION
The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae
- Authors
- Di Rosa, V., Frigato, E., López-Olmeda, J.F., Sánchez-Vázquez, F.J., Bertolucci, C.
- ID
- ZDB-PUB-150707-2
- Date
- 2015
- Source
- PLoS One 10: e0132235 (Journal)
- Registered Authors
- Bertolucci, Cristiano, Frigato, Elena
- Keywords
- Larvae, zebrafish, light, embryos, circadian rhythms, biological locomotion, gene expression, morphogenesis
- MeSH Terms
-
- Animals
- CLOCK Proteins/genetics*
- Circadian Clocks/genetics
- Circadian Clocks/radiation effects*
- Circadian Rhythm/genetics
- Circadian Rhythm/radiation effects*
- Gene Expression/radiation effects*
- Light*
- Motor Activity/genetics
- Motor Activity/radiation effects*
- Photoperiod
- Zebrafish
- PubMed
- 26147202 Full text @ PLoS One
Citation
Di Rosa, V., Frigato, E., López-Olmeda, J.F., Sánchez-Vázquez, F.J., Bertolucci, C. (2015) The Light Wavelength Affects the Ontogeny of Clock Gene Expression and Activity Rhythms in Zebrafish Larvae. PLoS One. 10:e0132235.
Abstract
Light plays a key role in synchronizing rhythms and setting the phase of early development. However, to date, little is known about the impact of light wavelengths during the ontogeny of the molecular clock and the behavioural rhythmicity. The aim of this research was to determine the effect of light of different wavelengths (white, blue and red) on the onset of locomotor activity and clock gene (per1b, per2, clock1, bmal1 and dbp) expression rhythms. For this purpose, 4 groups of zebrafish embryo/larvae were raised from 0 to 7 days post-fertilization (dpf) under the following lighting conditions: three groups maintained under light:dark (LD) cycles with white (full visible spectrum, LDW), blue (LDB), or red light (LDR), and one group raised under constant darkness (DD). The results showed that lighting conditions influenced activity rhythms. Larvae were arrhythmic under DD, while under LD cycles they developed wavelength-dependent daily activity rhythms which appeared earlier under LDB (4 dpf) than under LDW or LDR (5 dpf). The results also revealed that development and lighting conditions influenced clock gene expression. While clock1 rhythmic expression appeared in all lighting conditions at 7 dpf, per1b, per2 and dbp showed daily variations already at 3 dpf. Curiously, bmal1 showed consistent rhythmic expression from embryonic stage (0 dpf). Summarizing, the data revealed that daily rhythms appeared earlier in the larvae reared under LDB than in those reared under LDW and LDR. These results emphasize the importance of lighting conditions and wavelengths during early development for the ontogeny of daily rhythms of gene expression and how these rhythms are reflected on the behavioural rhythmicity of zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping