ZFIN ID: ZDB-PUB-150706-3
A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish
Riemer, S., Bontems, F., Krishnakumar, P., Gömann, J., Dosch, R.
Date: 2015
Source: Gene expression patterns : GEP   18(1-2): 44-52 (Journal)
Registered Authors: Bontems, Franck, Dosch, Roland, Krishnakumar, Pritesh, Riemer, Stephan
Keywords: Balbiani body, Bucky ball, Vasa, germline development, primordial germ cell, transgene
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Antibodies/metabolism
  • Germ Cells/metabolism*
  • Green Fluorescent Proteins/genetics*
  • Larva/genetics
  • Larva/growth & development
  • Larva/metabolism
  • Oocytes/cytology
  • Oocytes/metabolism
  • Oogenesis
  • Recombinant Fusion Proteins/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism
PubMed: 26143227 Full text @ Gene Expr. Patterns
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ABSTRACT
In many animals, the germline is specified by maternal RNA-granules termed germ plasm. The correct localization of germ plasm during embryogenesis is therefore crucial for the specification of germ cells. In zebrafish, we previously identified Bucky ball (Buc) as a key regulator of germ plasm formation. Here, we used a Buc antibody to describe its continuous germ plasm localization. Moreover, we generated a transgenic Buc-GFP line for live imaging, which visualizes germ plasm from its assembly during oogenesis up to the larval stages. Live imaging of Buc-GFP generated stunning movies, as they highlighted the dynamic details of germ plasm movements. Moreover, we discovered that Buc was still detected in primordial germ cells two days after fertilization. Interestingly, the transgene rescued buc mutants demonstrating genetically that the Buc-GFP fusion protein is functional. These results show that Buc-GFP exerts all biochemical interactions essential for germline development and highlight the potential of this line to analyze the molecular regulation of germ plasm formation.
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