PUBLICATION
A Fluorescence-Based Assay for Proteinuria Screening in Larval Zebrafish (Danio rerio)
- Authors
- Hanke, N., King, B.L., Vaske, B., Haller, H., Schiffer, M.
- ID
- ZDB-PUB-150701-2
- Date
- 2015
- Source
- Zebrafish 12(5): 372-6 (Other)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Biomarkers
- Dextrans/metabolism*
- Eye/metabolism*
- Green Fluorescent Proteins/metabolism
- Models, Animal*
- Proteinuria/genetics*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 26125680 Full text @ Zebrafish
Citation
Hanke, N., King, B.L., Vaske, B., Haller, H., Schiffer, M. (2015) A Fluorescence-Based Assay for Proteinuria Screening in Larval Zebrafish (Danio rerio). Zebrafish. 12(5):372-6.
Abstract
Analysis of genes compromising the glomerular filtration barrier in rodent models using transgenic or knockdown approaches is time- and resource-consuming and often leads to unsatisfactory results. Therefore, it would be beneficial to have a selection tool indicating that your gene of interest is in fact associated with proteinuria. Zebrafish (Danio rerio) is a rapid screening tool to study effects in glomerular filtration barrier integrity after genetic manipulation. We use either injection of high-molecular-weight dextrans or a transgenic fluorescent fish line [Tg(l-fabp:DBP:EGFP)] expressing a vitamin D-binding protein fused with eGFP for indirect detection of proteinuria. A loss of high-molecular-weight proteins from the circulation of the fish into the urine can be identified by monitoring fluorescence intensity in the zebrafish eye. Paired with an optimized analysis method, this assay provides an effective screening solution to detect filtration barrier damage with proteinuria before moving to a mammalian system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping