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ZFIN ID: ZDB-PUB-150623-3
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
Yu, D., Baird, M.A., Allen, J.R., Howe, E.S., Klassen, M.P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.Q., Weiner, O.D., Kornberg, T.B., Jan, Y.N., Davidson, M.W., Shu, X.
Date: 2015
Source: Nature Methods   12(8): 763-5 (Journal)
Registered Authors: Reade, Anna
Keywords: Fluorescence imaging, Fluorescent proteins, Cellular imaging, Transgenic organisms
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • DNA/chemistry
  • Developmental Biology
  • Drosophila melanogaster
  • Fluorescent Dyes/chemistry
  • Green Fluorescent Proteins/chemistry*
  • HeLa Cells
  • Histidine/chemistry
  • Humans
  • Infrared Rays*
  • Luminescent Proteins/chemistry
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Neurons/metabolism
  • Plasmids/metabolism
  • Protein Conformation
  • Protein Multimerization
  • Recombinant Fusion Proteins/chemistry
  • Transfection
  • Zebrafish
PubMed: 26098020 Full text @ Nat. Methods
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.