PUBLICATION
2-bromopalmitate impairs neural stem/progenitor cell proliferation, promotes cell apoptosis and induces malformation in zebrafish embryonic brain
- Authors
- Wang, C., Chen, X., Shi, W., Wang, F., Du, Z., Li, X., Yao, Y., Liu, T., Shao, T., Li, G., Hao, A.
- ID
- ZDB-PUB-150610-3
- Date
- 2015
- Source
- Neurotoxicology and teratology 50: 53-63 (Journal)
- Registered Authors
- Keywords
- 2-bromopalmitate, FGF signaling pathway, neural development, neural stem/progenitor cells, palmitoylation, zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/drug effects*
- Brain/drug effects*
- Brain/pathology
- Cell Proliferation/drug effects*
- Embryo, Nonmammalian/drug effects
- MAP Kinase Signaling System/drug effects
- Neural Stem Cells/drug effects*
- Palmitates/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 26056731 Full text @ Neurotoxicol. Teratol.
Citation
Wang, C., Chen, X., Shi, W., Wang, F., Du, Z., Li, X., Yao, Y., Liu, T., Shao, T., Li, G., Hao, A. (2015) 2-bromopalmitate impairs neural stem/progenitor cell proliferation, promotes cell apoptosis and induces malformation in zebrafish embryonic brain. Neurotoxicology and teratology. 50:53-63.
Abstract
2-bromopalmitate (2BP) is a widely used palmitoylation inhibitor. Besides, it has been reported that 2BP can inhibit T-cell activation, making it a potential immunosuppressor for treatment of autoimmune diseases. Although the important roles of palmitoylation in neural system have been noted during past decades, the effect of 2BP on neural development is still not very clear. In this study, we demonstrated that 25uM-100uM 2BP exposure caused apparent neural malformation in the presumptive brains of zebrafish embryos at 14 hpf. Further studies implied that the mRNA quantities and distributions of neural stem/progenitor cell (NSPC) markers (neurog1, sox2, sox3) in the affected regions of 50uM 2BP treated embryos significantly decreased. In addition, we found that 2BP impaired the NSPC proliferation at 10 hpf and 14 hpf as well as promoted cell apoptosis at 14 hpf, consistent with which the interference with FGF/ERK signaling pathway was also detected. For the first time, this study provided information about the toxicity and teratogenicity of 2BP for neural development in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping