PUBLICATION
Toxic effects of perfluorononanoic acid on the development of Zebrafish (Danio rerio) embryos
- Authors
- Liu, H., Sheng, N., Zhang, W., Dai, J.
- ID
- ZDB-PUB-150605-7
- Date
- 2015
- Source
- Journal of environmental sciences (China) 32: 26-34 (Journal)
- Registered Authors
- Zhang, Wei
- Keywords
- Danio rerio, Developmental toxicity, Perfluorononanoic acid, Zebrafish embryo
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects*
- Embryonic Development/genetics
- Fluorocarbons/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Lipid Metabolism/drug effects
- Lipid Metabolism/genetics
- Oxidative Stress/drug effects
- Oxidative Stress/genetics
- Reactive Oxygen Species/metabolism
- Real-Time Polymerase Chain Reaction
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 26040728 Full text @ J. Environ. Sci. (China).
Citation
Liu, H., Sheng, N., Zhang, W., Dai, J. (2015) Toxic effects of perfluorononanoic acid on the development of Zebrafish (Danio rerio) embryos. Journal of environmental sciences (China). 32:26-34.
Abstract
Perfluorononanoic acid (PFNA) is a nine-carbon perfluoroalkyl acid widely used in industrial and domestic products. It is a persistent organic pollutant found in the environment as well as in the tissues of humans and wildlife. There is a concern that this chemical might be a developmental toxicant and teratogen in various ecosystems. In the present study, the toxic effects of PFNA were evaluated in zebrafish (Danio rerio) embryos. One hour post-fertilization embryos were treated with 0, 25, 50, 100, 200, 300, 350, and 400μmol/L PFNA for 96hr in 6-well plates. Developmental phenotypes and hatching rates were observed and recorded. Nineteen genes related to oxidative stress and lipid metabolism were examined using Quantitative RT-PCR and confirmed by whole mount in situ hybridization (WISH). Results showed that PFNA delayed the development of zebrafish embryos, reduced the hatching rate, and caused ventricular edema and malformation of the spine. In addition, the amount of reactive oxygen species in the embryo bodies increased significantly after exposure to PFNA compared with that of the control group. The Quantitative RT-PCR and WISH experiments demonstrated that mRNA expression of the lfabp and ucp2 genes increased significantly while that of sod1 and mt-nd1 decreased significantly after PFNA exposure. The mRNA expression levels of gpx1 and mt-atp6 decreased significantly in the high concentration group. However, the mRNA expression levels of both ppara and pparg did not show any significant variation after exposure. These findings suggest that PFNA affected the development of zebrafish embryos at relatively low concentrations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping