ZFIN ID: ZDB-PUB-150605-3
The Chromatin Remodeling Protein Bptf Promotes Posterior Neuroectodermal Fate by Enhancing Smad2-Activated wnt8a Expression
Ma, Y., Liu, X., Liu, Z., Wei, S., Shang, H., Xue, Y., Cao, Y., Meng, A., Wang, Q.
Date: 2015
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience   35: 8493-506 (Journal)
Registered Authors: Meng, Anming, Wang, Qiang
Keywords: bptf, neural posteriorization, nucleosome remodeling, smad2, wnt8a
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Antigens, Nuclear/genetics
  • Antigens, Nuclear/metabolism*
  • Benzamides
  • Cytoskeletal Proteins/metabolism*
  • Dioxoles
  • Embryo, Mammalian/drug effects
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Developmental/genetics*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Immunoprecipitation
  • Male
  • Mutation/genetics
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism*
  • Neural Plate/embryology*
  • Neural Plate/metabolism*
  • Oligodeoxyribonucleotides, Antisense
  • RNA, Messenger/metabolism
  • Receptors, Transforming Growth Factor beta/antagonists & inhibitors
  • Smad2 Protein/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Tumor Suppressor Protein p53/genetics
  • Tumor Suppressor Protein p53/metabolism
  • Wnt Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 26041917 Full text @ J. Neurosci.
During vertebrate embryogenesis, the neuroectoderm is induced from dorsal ectoderm and then partitioned into anterior and posterior neuroectodermal domains by posteriorizing signals, such as Wnt and fibroblast growth factor. However, little is known about epigenetic regulation of posteriorizing gene expression. Here, we report a requirement of the chromatin remodeling protein Bptf for neuroectodermal posteriorization in zebrafish embryos. Knockdown of bptf leads to an expansion of the anterior neuroectoderm at the expense of the posterior ectoderm. Bptf functionally and physically interacts with p-Smad2, which is activated by non-Nodal TGF-β signaling, to promote the expression of wnt8a, a critical gene for neural posteriorization. Bptf and Smad2 directly bind to and activate the wnt8a promoter through recruiting NURF remodeling complex. When bptf function or TGF-β signal transduction is inhibited, the nucleosome density on the wnt8a promoter is increased. We propose that Bptf and TGF-β/Smad2 mediate nucleosome remodeling to regulate wnt8a expression and hence neural posteriorization.