PUBLICATION
Anti-oxidative responses of zebrafish (Danio rerio) gill, liver and brain tissues upon acute cold shock
- Authors
- Wu, S.M., Liu, J.H., Shu, L.H., Chen, C.H.
- ID
- ZDB-PUB-150531-1
- Date
- 2015
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 187: 202-13 (Journal)
- Registered Authors
- Keywords
- Apoptosis, brain, cold shock, gills, liver, metallothionein, oxidative stress, zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/metabolism*
- Apoptosis
- Brain/physiology*
- Cold Temperature*
- Gills/physiology*
- Lipid Peroxidation
- Liver/physiology*
- Reactive Oxygen Species/metabolism
- Stress, Physiological*
- Zebrafish/physiology*
- PubMed
- 26025641 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Wu, S.M., Liu, J.H., Shu, L.H., Chen, C.H. (2015) Anti-oxidative responses of zebrafish (Danio rerio) gill, liver and brain tissues upon acute cold shock. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 187:202-13.
Abstract
The present study seeks to detect oxidative damage and to compare anti-oxidative responses among liver, gills and brain of adult zebrafish were cooled from 28°C (control) to 12°C (treatment) for 0-24 h. The lipid peroxidation (LPO) of liver, gill and brain tissue significantly increased at 1 h after transfer, but reactive oxygen species (ROS) in the treatment group increased significantly after 24 h as compared to the control. The fish were found to develop a cascading anti-oxidative mechanism beginning with an increase in Cu/Zn-SOD levels, followed by increased CAT and GPx mRNA expressions in the three tissue types. Both smtB and mt2 mRNA increased in the hepatic and brain tissues following 1 h of cold stress, but only smtB exhibited a significant increase in the gills at 1 and 6 h after transfer to 12°C. Furthermore, cellular apoptosis in the brain was not evident after cold shock, but liver and gills showed cellular apoptosis at 1-3 h, with another peak in the liver at 6 h after cold shock. The results suggest that the cold shock induced oxidative stress, and the enzymatic (SOD, GPx and CAT) and non-enzymatic (mt-2 and smt-B) mRNA expression all play a role in the resulting anti-oxidation within 1-6 h of cold shock. A functional comparison showed that the brain had the most powerful antioxidant defense system of the three tissue types since it had the highest smtB mRNA expression and a lower level of cell apoptosis than the liver and gills after exposure to cold stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping