PUBLICATION
Developmental toxicity and endocrine disruption of naphthenic acids on the early life stage of zebrafish (Danio rerio)
- Authors
- Wang, J., Cao, X., Huang, Y., Tang, X.
- ID
- ZDB-PUB-150523-15
- Date
- 2015
- Source
- Journal of applied toxicology : JAT 35(12): 1493-501 (Journal)
- Registered Authors
- Keywords
- CYP19, VTG, embryotoxicity, estrogen receptor, naphthenic acids
- MeSH Terms
-
- Animals
- Carboxylic Acids/toxicity*
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Endocrine Disruptors/toxicity*
- Endocrine System/drug effects*
- Endocrine System/embryology
- Gene Expression Regulation, Developmental/drug effects
- Oil and Gas Fields/chemistry
- Organogenesis/drug effects*
- Organogenesis/genetics
- Wastewater/chemistry
- Zebrafish/embryology*
- PubMed
- 25995127 Full text @ J. Appl. Toxicol.
- CTD
- 25995127
Citation
Wang, J., Cao, X., Huang, Y., Tang, X. (2015) Developmental toxicity and endocrine disruption of naphthenic acids on the early life stage of zebrafish (Danio rerio). Journal of applied toxicology : JAT. 35(12):1493-501.
Abstract
Oil sands process-affected water (OSPW) has been reported to exhibit adverse effects on the environment and wildlife. Although the compounds responsible are unknown, naphthenic acids (NAs) have been considered to be implicated. The current study was designed to investigate whether NAs might cause developmental toxicity and endocrine disruption on the early life stage of zebrafish (Danio rerio). The success of embryo hatch was inhibited by 2.5 mg l(-1) oil sands NAs (OS-NAs) exposure, and both OSPW NAs and commercial NAs (C-NAs) exposure resulted in a variety of developmental lesions in the fish larvae, such as yolk sac edema, pericardial edema and spinal malformation. The transcription of genes involved cytochrome P450 aromatase (CYP19a and CYP19b), estrogen receptors (ERα, ERβ1 and ERβ2), and vitellogenin (VTG) was analyzed to evaluate the endocrine disrupting effects of NAs. Significant up-regulated gene expressions of CYP19b, ERα and VTG were observed in both OS-NAs and C-NAs groups, which indicated the deleteriously estrogenic potential of NAs. These results confirmed that NAs derived from crude petroleum could negatively impact the development and endocrine function of zebrafish, and be primarily responsible for the toxicity of OSPW.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping