PUBLICATION
A structural and genotypic scaffold underlying temporal integration
- Authors
- Lee, M.M., Arrenberg, A.B., Aksay, E.R.
- ID
- ZDB-PUB-150523-11
- Date
- 2015
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 35: 7903-20 (Journal)
- Registered Authors
- Keywords
- eye movements, microcircuit, neural integrator, patterning, persistent activity, temporal integration
- MeSH Terms
-
- Animals
- Eye Movements*
- Eye Proteins/metabolism
- Female
- GABAergic Neurons/classification
- GABAergic Neurons/metabolism
- GABAergic Neurons/physiology*
- Genotype*
- Homeodomain Proteins/metabolism
- Male
- Motor Neurons/classification
- Motor Neurons/metabolism
- Motor Neurons/physiology*
- Rhombencephalon/cytology
- Rhombencephalon/metabolism
- Rhombencephalon/physiology*
- Transcription Factors/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 25995475 Full text @ J. Neurosci.
Citation
Lee, M.M., Arrenberg, A.B., Aksay, E.R. (2015) A structural and genotypic scaffold underlying temporal integration. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:7903-20.
Abstract
The accumulation and storage of information over time, temporal integration, is key to numerous behaviors. Many oculomotor tasks depend on integration of eye-velocity signals to eye-position commands, a transformation achieved by a hindbrain cell group termed the velocity-to-position neural integrator (VPNI). Although the VPNI's coding properties have been well characterized, its mechanism of function remains poorly understood because few links exist between neuronal activity, structure, and genotypic identity. To fill this gap, we used calcium imaging and single-cell electroporation during oculomotor behaviors to map VPNI neural activity in zebrafish onto a hindbrain scaffold consisting of alternating excitatory and inhibitory parasagittal stripes. Three distinct classes of VPNI cells were identified. One glutamatergic class was medially located along a stripe associated with the alx transcription factor; these cells had ipsilateral projections terminating near abducens motoneurons and collateralized extensively within the ipsilateral VPNI in a manner consistent with integration through recurrent excitation. A second glutamatergic class was more laterally located along a stripe associated with transcription factor dbx1b; these glutamatergic cells had contralateral projections collateralizing near abducens motoneurons, consistent with a role in disconjugate eye movements. A third class, immunohistochemically suggested to be GABAergic, was located primarily in the dbx1b stripe and also had contralateral projections terminating near abducens motoneurons; these cells collateralized extensively in the dendritic field of contralateral VPNI neurons, consistent with a role in coordinating activity between functionally opposing populations. This mapping between VPNI activity, structure, and genotype may provide a blueprint for understanding the mechanisms governing temporal integration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping