PUBLICATION
In vivo single-cell labeling by confined primed conversion
- Authors
- Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., Pantazis, P.
- ID
- ZDB-PUB-150520-7
- Date
- 2015
- Source
- Nature Methods 12(7): 645-8 (Journal)
- Registered Authors
- Dempsey, William, Pantazis, Periklis (Laki)
- Keywords
- none
- MeSH Terms
-
- Animals
- Microscopy, Fluorescence, Multiphoton/methods*
- Neurons/cytology*
- Zebrafish
- PubMed
- 25984699 Full text @ Nat. Methods
Citation
Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., Pantazis, P. (2015) In vivo single-cell labeling by confined primed conversion. Nature Methods. 12(7):645-8.
Abstract
Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping