ZFIN ID: ZDB-PUB-150520-15
Ribonuclease like 5 regulates zebrafish yolk extension by suppressing a p53-dependent DNA damage response pathway
Zhai, Y., Zhao, X., Sheng, J., Gao, X., Ou, Z., Xu, Z.
Date: 2015
Source: The international journal of biochemistry & cell biology 65: 12-19 (Journal)
Registered Authors:
Keywords: DNA damage, Embryogenesis, P53, Ribonuclease like 5, Yolk extension
MeSH Terms:
  • Animals
  • DNA Damage*
  • Embryo, Nonmammalian
  • Embryonic Development/physiology
  • Gene Knockdown Techniques
  • Ribonuclease, Pancreatic/genetics
  • Ribonuclease, Pancreatic/metabolism*
  • Tumor Suppressor Protein p53/genetics*
  • Tumor Suppressor Protein p53/metabolism
  • Yolk Sac/growth & development
  • Yolk Sac/metabolism
  • Yolk Sac/physiology*
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 25980932 Full text @ Int. J. Biochem. Cell Biol.
Ribonuclease like 5 (Rnasel5) is a novel member of the zebrafish ribonuclease A family and its expression is increased during early embryogenesis. However, the in vivo biological function of Rnasel5 remains to be elucidated. Here, we report that knockdown of Rnasel5 by morhpolinos caused shrunken yolk extension as well as increased DNA damage at yolk syncytial layer and external tissue layers via the activation of p53 pathway. In addition, the morphological defects caused by Rnasel5 knockdown can be partially rescued by mRNA injection. Our findings provide the first functional characterization of Rnasel5 in zebrafish development and reveal its critical role in yolk extension by modulation of the p53 pathway.