Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92 cluster in endothelial cells.
- Authors
- Kaluza, D., Kroll, J., Gesierich, S., Manavski, Y., Boeckel, J., Doebele, C., Zelent, A., Rossig, L., Zeiher, A. M., Augustin, H. G., Urbich, C., Dimmeler, S.
- ID
- ZDB-PUB-150422-15
- Date
- 2013
- Source
- Arteriosclerosis, Thrombosis, and Vascular Biology 33: 533-543 (Journal)
- Registered Authors
- Kroll, Jens
- Keywords
- Angiogenesis, Histone deacetylase 9 and histone deacetylase 5, Histone deacetylases, MicroRNAs, miR-17
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Gene Knockdown Techniques
- HEK293 Cells
- Hindlimb
- Histone Deacetylases/deficiency
- Histone Deacetylases/genetics
- Histone Deacetylases/metabolism*
- Human Umbilical Vein Endothelial Cells/enzymology*
- Humans
- Ischemia/enzymology*
- Ischemia/genetics
- Ischemia/physiopathology
- Mice
- Mice, Knockout
- MicroRNAs/genetics
- MicroRNAs/metabolism*
- Muscle, Skeletal/blood supply*
- Mutation
- Neovascularization, Physiologic*/genetics
- RNA Interference
- Regional Blood Flow
- Repressor Proteins/deficiency
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Retinal Neovascularization/enzymology*
- Retinal Neovascularization/genetics
- Retinal Neovascularization/physiopathology
- Transfection
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 23288173 Full text @ Arterio., Thromb., and Vas. Bio.
Abstract
Objective—Histone deacetylases (HDACs) modulate gene expression by deacetylation of histone and nonhistone proteins. Several HDACs control angiogenesis, but the role of HDAC9 is unclear.
Methods and Results—Here, we analyzed the function of HDAC9 in angiogenesis and its involvement in regulating microRNAs. In vitro, silencing of HDAC9 reduces endothelial cell tube formation and sprouting. Furthermore, HDAC9 silencing decreases vessel formation in a spheroid-based Matrigel plug assay in mice and disturbs vascular patterning in zebrafish embryos. Genetic deletion of HDAC9 reduces retinal vessel outgrowth and impairs blood flow recovery after hindlimb ischemia. Consistently, overexpression of HDAC9 increases endothelial cell sprouting, whereas mutant constructs lacking the catalytic domain, the nuclear localization sequence, or sumoylation site show no effect. To determine the mechanism underlying the proangiogenic effect of HDAC9, we measured the expression of the microRNA (miR)-17–92 cluster, which is known for its antiangiogenic activity. We demonstrate that silencing of HDAC9 in endothelial cells increases the expression of miR-17–92. Inhibition of miR-17–20a rescues the sprouting defects induced by HDAC9 silencing in vitro and blocking miR-17 expression partially reverses the disturbed vascular patterning of HDAC9 knockdown in zebrafish embryos.
Conclusion—We found that HDAC9 promotes angiogenesis and transcriptionally represses the miR-17–92 cluster.