PUBLICATION
Chemical genetic effects of Sargassum wightii during embryonic development in zebrafish
- Authors
- Kannan, R.R., Iniyan, A.M., Vincent, S.G.
- ID
- ZDB-PUB-150417-6
- Date
- 2015
- Source
- Indian journal of pharmacology 47: 195-8 (Journal)
- Registered Authors
- Keywords
- Chemical genetics, phytomolecules, small molecules screening, tubular heart, zebrafish
- MeSH Terms
-
- Abnormalities, Drug-Induced/embryology
- Abnormalities, Drug-Induced/etiology*
- Animals
- Biological Products/isolation & purification
- Biological Products/toxicity*
- Embryo, Nonmammalian/drug effects*
- Embryonic Development*/drug effects
- Embryonic Development*/genetics
- Mutation
- Pericardium/abnormalities
- Pericardium/drug effects
- Pericardium/embryology
- Sargassum/chemistry*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 25878381 Full text @ Indian J Pharmacol
Citation
Kannan, R.R., Iniyan, A.M., Vincent, S.G. (2015) Chemical genetic effects of Sargassum wightii during embryonic development in zebrafish. Indian journal of pharmacology. 47:195-8.
Abstract
Objective Phenotype based small molecule discovery is a category of chemical genetic study. The aim of this study was to observe the phytochemical based genetic effects of Sargassum wightii during organogenesis in embryonic zebrafish.
Materials and methods The phytomolecules from S. wightii were extracted using organic solvents and treated with the 24 h old developing zebrafish embryos. The active extract was partially purified by column chromatography, C18 Sep-Pak column and reversed-phase high-performance liquid chromatography.
Results Initially, cardiac bulging was found in 2 dpf to 3 dpf (days post fertilization), then bradycardia and tubular heart were observed in the next 8 h, which also showed the reduction in the heart beat rates. The phenotypic mutation effects of bre, has, dou yan, heg and you were observed in the 3 dpf and 4 dpf of the extract treated zebrafish embryos.
Conclusions This study demonstrated that the phytomolecules from S. wightii exhibited potential molecular switches on the developmental process, which might have significant role in understanding the development based chemical genetic studies in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping