PUBLICATION

Neuronal activity biases axon selection for myelination in vivo

Authors
Hines, J.H., Ravanelli, A.M., Schwindt, R., Scott, E.K., Appel, B.
ID
ZDB-PUB-150408-1
Date
2015
Source
Nature Neuroscience   18(5): 683-9 (Journal)
Registered Authors
Appel, Bruce, Ravanelli, Andrew M., Scott, Ethan
Keywords
none
MeSH Terms
  • Action Potentials/physiology*
  • Animals
  • Animals, Genetically Modified
  • Axons/ultrastructure*
  • Exocytosis/drug effects
  • Genes, Reporter
  • Green Fluorescent Proteins/analysis
  • Green Fluorescent Proteins/genetics
  • Homeodomain Proteins/analysis
  • Homeodomain Proteins/genetics
  • Microscopy, Confocal
  • Myelin Sheath/drug effects
  • Myelin Sheath/physiology*
  • Neurogenesis
  • Oligodendroglia/physiology*
  • Potassium Channels, Inwardly Rectifying/genetics
  • Potassium Channels, Inwardly Rectifying/physiology
  • Recombinant Fusion Proteins/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/drug effects
  • Spinal Cord/growth & development
  • Synaptic Vesicles/drug effects
  • Synaptic Vesicles/physiology
  • Tetrodotoxin/pharmacology
  • Time-Lapse Imaging
  • Transcription Factors/analysis
  • Transcription Factors/genetics
  • Veratridine/pharmacology
  • Zebrafish/embryology
PubMed
25849987 Full text @ Nat. Neurosci.
Abstract
An essential feature of vertebrate neural development is ensheathment of axons with myelin, an insulating membrane formed by oligodendrocytes. Not all axons are myelinated, but mechanisms directing myelination of specific axons are unknown. Using zebrafish, we found that activity-dependent secretion stabilized myelin sheath formation on select axons. When VAMP2-dependent exocytosis was silenced in single axons, oligodendrocytes preferentially ensheathed neighboring axons. Nascent sheaths formed on silenced axons were shorter in length, but when activity of neighboring axons was also suppressed, inhibition of sheath growth was relieved. Using in vivo time-lapse microscopy, we found that only 25% of oligodendrocyte processes that initiated axon wrapping were stabilized during normal development and that initiation did not require activity. Instead, oligodendrocyte processes wrapping silenced axons retracted more frequently. We propose that axon selection for myelination results from excessive and indiscriminate initiation of wrapping followed by refinement that is biased by activity-dependent secretion from axons.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes