ZFIN ID: ZDB-PUB-150319-5
A PAX1 enhancer locus is associated with susceptibility to idiopathic scoliosis in females
Sharma, S., Londono, D., Eckalbar, W.L., Gao, X., Zhang, D., Mauldin, K., Kou, I., Takahashi, A., Matsumoto, M., Kamiya, N., Murphy, K.K., Cornelia, R., TSRHC Scoliosis Clinical Group, Japan Scoliosis Clinical Research Group, Herring, J.A., Burns, D., Ahituv, N., Ikegawa, S., Gordon, D., Wise, C.A.
Date: 2015
Source: Nature communications   6: 6452 (Journal)
Registered Authors: Ahituv, Nadav, Murphy, Karl
Keywords: none
MeSH Terms:
  • Alleles
  • Animals
  • Chromosome Mapping
  • Cohort Studies
  • Enhancer Elements, Genetic*
  • Female
  • Genetic Predisposition to Disease*
  • Genome-Wide Association Study
  • Genotype
  • Humans
  • Japan
  • Linkage Disequilibrium
  • Male
  • Mutation
  • Paired Box Transcription Factors/genetics*
  • Paired Box Transcription Factors/physiology
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Risk Factors
  • Scoliosis/genetics*
  • Sequence Analysis, DNA
  • Sex Factors
  • United States
  • Zebrafish
PubMed: 25784220 Full text @ Nat. Commun.
Idiopathic scoliosis (IS) is a common paediatric musculoskeletal disease that displays a strong female bias. By performing a genome-wide association study (GWAS) of 3,102 individuals, we identify significant associations with 20p11.22 SNPs for females (P=6.89 × 10(-9)) but not males (P=0.71). This association with IS is also found in independent female cohorts from the United States of America and Japan (overall P=2.15 × 10(-10), OR=1.30 (rs6137473)). Unexpectedly, the 20p11.22 IS risk alleles were previously associated with protection from early-onset alopecia, another sexually dimorphic condition. The 174-kb associated locus is distal to PAX1, which encodes paired box 1, a transcription factor involved in spine development. We identify a sequence in the associated locus with enhancer activity in zebrafish somitic muscle and spinal cord, an activity that is abolished by IS-associated SNPs. We thus identify a sexually dimorphic IS susceptibility locus, and propose the first functionally defined candidate mutations in an enhancer that may regulate expression in specific spinal cells.