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ZFIN ID: ZDB-PUB-150311-17
Neuroendocrine regulation of the stress response in adult zebrafish, Danio rerio
Pavlidis, M., Theodoridi, A., Tsalafouta, A.
Date: 2015
Source: Progress in neuro-psychopharmacology & biological psychiatry 60: 121-31 (Journal)
Registered Authors:
Keywords: Cortisol, Stress, Zebrafish, brain-derived neurotrophic factor (bdnf)
MeSH Terms:
  • Analysis of Variance
  • Animals
  • Disease Models, Animal
  • Hydrocortisone/metabolism*
  • Hypothalamic Hormones/genetics
  • Hypothalamic Hormones/metabolism*
  • Neurosecretory Systems/metabolism*
  • RNA, Messenger/metabolism
  • Stress, Psychological/metabolism*
  • Time Factors
  • Zebrafish
PubMed: 25748166 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
The main objectives of this study were to investigate the dynamics of the cortisol stress response and the underlying molecular regulation in adult zebrafish exposed to acute and long-term stressors that differed in nature, duration and relative intensity. Fish showed a very rapid and prolonged increase in trunk cortisol concentrations, starting at around 15 min and returning to basal levels at around 2 h following exposure to acute stressors. In addition, acute stress affected significantly brain mRNA expression levels of several genes (corticotropin-releasing factor, crf; pro-opiomelanocortin , pomc; glucorticoid receptor, gr; MR/GR ratio; prolactin, prl; hypocretin/orexin, hcrt; brain-derived neurotrophic factor, bdnf; c-fos). Exposure of fish to unpredictable relatively low-grade environmental and husbandry stressors (SP-1) did not affect the overall behaviour of fish, as well as trunk cortisol concentrations. Fish exposed to relatively higher-grade long-term stressors (SP-2) showed elevated cortisol levels as well significant changes in most of gene transcripts. In particular, fish exposed to SP-2 showed statistically significant upregulation in brain gr, mr, prl and hcrt compared to SP-1 and control individuals. The highest mean values of bdnf transcripts were found in SP-2 exposed zebrafish and the lowest in control fish, while an approximately 5 to 6-fold upregulation was observed in c-fos mean relative mRNA levels of long-term stress-exposed fish, regardless of stressor intensity, compared to control zebrafish. In conclusion, we developed realistic acute and unpredictable long-term stress protocols, based on husbandry and environmental stressors and physical, chemical, mechanical and social stimuli that fish may experience either in nature or under intensive rearing conditions.