header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-150311-17
Neuroendocrine regulation of the stress response in adult zebrafish, Danio rerio
Pavlidis, M., Theodoridi, A., Tsalafouta, A.
Date: 2015
Source: Progress in neuro-psychopharmacology & biological psychiatry 60: 121-31 (Journal)
Registered Authors:
Keywords: Cortisol, Stress, Zebrafish, brain-derived neurotrophic factor (bdnf)
MeSH Terms:
  • Analysis of Variance
  • Animals
  • Disease Models, Animal
  • Hydrocortisone/metabolism*
  • Hypothalamic Hormones/genetics
  • Hypothalamic Hormones/metabolism*
  • Neurosecretory Systems/metabolism*
  • RNA, Messenger/metabolism
  • Stress, Psychological/metabolism*
  • Time Factors
  • Zebrafish
PubMed: 25748166 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
FIGURES
ABSTRACT
The main objectives of this study were to investigate the dynamics of the cortisol stress response and the underlying molecular regulation in adult zebrafish exposed to acute and long-term stressors that differed in nature, duration and relative intensity. Fish showed a very rapid and prolonged increase in trunk cortisol concentrations, starting at around 15 min and returning to basal levels at around 2 h following exposure to acute stressors. In addition, acute stress affected significantly brain mRNA expression levels of several genes (corticotropin-releasing factor, crf; pro-opiomelanocortin , pomc; glucorticoid receptor, gr; MR/GR ratio; prolactin, prl; hypocretin/orexin, hcrt; brain-derived neurotrophic factor, bdnf; c-fos). Exposure of fish to unpredictable relatively low-grade environmental and husbandry stressors (SP-1) did not affect the overall behaviour of fish, as well as trunk cortisol concentrations. Fish exposed to relatively higher-grade long-term stressors (SP-2) showed elevated cortisol levels as well significant changes in most of gene transcripts. In particular, fish exposed to SP-2 showed statistically significant upregulation in brain gr, mr, prl and hcrt compared to SP-1 and control individuals. The highest mean values of bdnf transcripts were found in SP-2 exposed zebrafish and the lowest in control fish, while an approximately 5 to 6-fold upregulation was observed in c-fos mean relative mRNA levels of long-term stress-exposed fish, regardless of stressor intensity, compared to control zebrafish. In conclusion, we developed realistic acute and unpredictable long-term stress protocols, based on husbandry and environmental stressors and physical, chemical, mechanical and social stimuli that fish may experience either in nature or under intensive rearing conditions.
ADDITIONAL INFORMATION