PUBLICATION
Long-term effects of Bisphenol AF (BPAF) on hormonal balance and genes of hypothalamus-pituitary-gonad axis and liver of zebrafish (Danio rerio), and the impact on offspring
- Authors
- Shi, J., Jiao, Z., Zheng, S., Li, M., Zhang, J., Feng, Y., Yin, J., Shao, B.
- ID
- ZDB-PUB-150301-10
- Date
- 2015
- Source
- Chemosphere 128C: 252-257 (Journal)
- Registered Authors
- Zhang, Jing
- Keywords
- Bisphenol AF, Hypothalamus-pituitary-gonad (HPG) axis, Steroid hormonal balance, Steroidgenesis, Zebrafish
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Endocrine System/drug effects
- Estradiol/metabolism
- Female
- Gonads/drug effects*
- Hypothalamo-Hypophyseal System/drug effects*
- Hypothalamus/metabolism
- Liver/metabolism*
- Male
- Phenols/toxicity*
- Time Factors
- Vitellogenins/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- PubMed
- 25723718 Full text @ Chemosphere
- CTD
- 25723718
Citation
Shi, J., Jiao, Z., Zheng, S., Li, M., Zhang, J., Feng, Y., Yin, J., Shao, B. (2015) Long-term effects of Bisphenol AF (BPAF) on hormonal balance and genes of hypothalamus-pituitary-gonad axis and liver of zebrafish (Danio rerio), and the impact on offspring. Chemosphere. 128C:252-257.
Abstract
Bisphenol AF (BPAF) is one of the analogues of bisphenol A (BPA) and is widely used as a raw material in the plastics industry. The potential toxicity to fish from exposure to BPAF in the aquatic environment is largely unknown. In this study, zebrafish (Danio rerio) were exposed to BPAF at 5, 25 and 125µgL(-1), from 4 hour-post-fertilization (hpf) to 120day-post-fertilization (dpf), representing the period from embryo to adult. The levels of plasma hormones were measured and the expression of selected representative genes along the hypothalamus-pituitary-gonad (HPG) axis and liver were examined. The concentration of 17β-estradiol (E2) was significantly increased in male and female fish and a significant decrease of testosterone (T) was observed in male fish. The mRNA expression of genes along the HPG axis and in liver tissues in F0 generation fish demonstrated that the steroid hormonal balances of zebrafish were modulated through the alteration of steroidgenesis. The significant decrease of egg fertilization among offspring indicates the possibility of sperm deterioration of parent following exposure to BPAF. The higher occurrence of malformation and lower survival rate in the offspring from the exposure group suggested a possibility of maternal transfer of BPAF, which could be responsible for the increased prevalence of adverse health signs in the offspring. The hatching delay in 5µgL(-1) BPAF indicated that parental exposure to environmentally relevant concentration of BPAF would result in delayed hatching of the offspring. A potential consequence of adverse effects in the offspring by BPAF deserves further investigation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping