PUBLICATION
Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos
- Authors
- Rodríguez-Fuentes, G., Sandoval-Gío, J.J., Arroyo-Silva, A., Noreña-Barroso, E., Escalante-Herrera, K.S., Olvera-Espinosa, F.
- ID
- ZDB-PUB-150211-8
- Date
- 2015
- Source
- Ecotoxicology and environmental safety 115C: 14-18 (Journal)
- Registered Authors
- Keywords
- 3-Benzophenone, Danio rerio, Oxidative stress, UV filters, Vitellogenin
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Benzophenones/analysis
- Benzophenones/toxicity*
- Catalase/genetics
- Catalase/metabolism
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Estrogens, Non-Steroidal/toxicity*
- Glutathione Peroxidase/genetics
- Glutathione Peroxidase/metabolism
- Oxidative Stress*
- Sunscreening Agents/analysis
- Sunscreening Agents/toxicity*
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- Vitellogenins/genetics
- Vitellogenins/metabolism
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 25666732 Full text @ Ecotoxicol. Environ. Saf.
Citation
Rodríguez-Fuentes, G., Sandoval-Gío, J.J., Arroyo-Silva, A., Noreña-Barroso, E., Escalante-Herrera, K.S., Olvera-Espinosa, F. (2015) Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos. Ecotoxicology and environmental safety. 115C:14-18.
Abstract
Personal care products have been detected in superficial waters, representing an environmental risk to the biota. Some studies indicated that 3-benzophenone (3BP) alters hormones, inducing vitellogenesis and having adverse effects on fish reproduction. Other studies have reported generation of free radicals and changes in antioxidant enzymes. Therefore, the aim of the present study was to test acute exposure to 3BP at concentrations within and beyond that found environmentally to provide important toxicological information regarding this chemical. We evaluated the effect of 3BP on vitellogenin 1 (VTG1) gene expression and the transcription of the enzymes catalase (CAT), superoxide dismutase (SOD) or glutathione peroxidase (GPx), which are involved in cellular redox balance. Zebrafish eluthero-embryos (168hpf) were exposed to 1,10, 100, 1000µg/L 3BP, in addition to a negative control and a 0.1% ethanol control for 48h. The results of our study indicated a positive significant correlation between exposure concentrations and VTG1 expression (r=0.986, p=0.0028) but only 1000µg/L 3BP produced a significant increase from control. Acute exposure showed no significant differences in transcription levels of CAT, SOD or GPx at the tested conditions. Nevertheless, a trend toward increase in GPx expression was observed as a positive significant correlation (r=0.928, p=0.017) was noted.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping