PUBLICATION
Two-Photon Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Persulfide and Polysulfide in Living Organisms
- Authors
- Zeng, L., Chen, S., Xia, T., Hu, W., Li, C., Liu, Z.
- ID
- ZDB-PUB-150207-9
- Date
- 2015
- Source
- Analytical chemistry 87(5): 3004-10 (Journal)
- Registered Authors
- Hu, Wei
- Keywords
- none
- MeSH Terms
-
- Animals
- Blotting, Western
- Cystathionine beta-Synthase/metabolism
- Cystathionine gamma-Lyase/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Fluorescent Dyes/chemistry*
- HeLa Cells
- Humans
- Hydrogen Sulfide/analysis*
- Image Processing, Computer-Assisted/methods*
- Photons*
- Sulfides/analysis*
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 25655109 Full text @ Anal. Chem.
Citation
Zeng, L., Chen, S., Xia, T., Hu, W., Li, C., Liu, Z. (2015) Two-Photon Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Persulfide and Polysulfide in Living Organisms. Analytical chemistry. 87(5):3004-10.
Abstract
Hydrogen persulfide and polysulfide (H2Sn) are the newly discovered intracellular reactive species considered to be with high protein S-sulfhydration efficiency. The detection of H2Sn in living systems is essential for studying their functions but quite challenging. In this work, we report a two-photon excited fluorescent probe, QSn, capable of tracing H2Sn in living organisms. QSn exhibited turn-on two-photon fluorescence response upon reaction with H2Sn. With favorable photophysical property, high specificity, and low cytotoxicity, QSn was able to recognize exogenous H2Sn in living cells. More importantly, it realized for the first time the visualization of endogenous H2Sn generated in cells overexpressing cystathionine β-synthase and cystathionine γ-lyase, the enzymes responsible for producing endogenous H2Sn. Taking advantages of two-photon microscopy, the probe was also applied to achieve H2Sn detection in zebrafish embryo and to observe the H2Sn distribution in living organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping