|ZFIN ID: ZDB-PUB-150201-4|
Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation
Hochgreb-Hägele, T., Koo, D.E., Bronner, M.E.
|Source:||Developmental Biology 400(1): 23-32 (Journal)|
|Registered Authors:||Bronner-Fraser, Marianne|
|Keywords:||Cartilage maturation, Neural crest, Ossification, Zebrafish, p53|
|PubMed:||25636963 Full text @ Dev. Biol.|
Hochgreb-Hägele, T., Koo, D.E., Bronner, M.E. (2015) Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation. Developmental Biology. 400(1):23-32.
ABSTRACTJaw formation involves an intricate series of molecular events, whereby a chondrogenic scaffold precedes osteogenesis. The mechanisms coupling timing of cartilage maturation to onset of bone differentiation are poorly understood, particularly for neural crest-derived bones of the head. Here we present a novel zebrafish gene/protein-trap Citrine-fusion line that reveals transient expression of the zinc-finger protein Znf385C in maturing chondrocytes of the jaw. Functional analysis shows that loss of Znf385C disrupts a distinct peak of p21(cip1/waf1) expression in the chondrocytes, as well as causes premature ossification of the zebrafish jaw. We find that Znf385C is expressed as two splice variants which act differentially to activate p21(cip1/waf1) and/or interact with p53 in subcellular compartments. Taken together, the results suggest that Znf385C acts as a developmental switch for p53 function that modulates cell cycle arrest of chondrocytes and regulates timing of jaw cartilage maturation and ossification.