ZFIN ID: ZDB-PUB-150127-8
Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish
Gut, P., Stainier, D.Y.
Date: 2015
Source: Methods in molecular biology (Clifton, N.J.)   1263: 157-165 (Other)
Registered Authors: Gut, Philipp, Stainier, Didier
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Drug Evaluation, Preclinical/methods*
  • Energy Metabolism/drug effects*
  • Fasting/metabolism*
  • Luminescent Measurements/methods
  • Small Molecule Libraries
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 25618343 Full text @ Meth. Mol. Biol.
Organismal energy homeostasis is maintained by complex interorgan communications making the discovery of novel drugs against metabolic diseases challenging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene phosphoenolpyruvate-carboxykinase 1 (pck1).