ZFIN ID: ZDB-PUB-150127-6
Zebrafish Rnf111 is encoded by multiple transcripts and is required for epiboly progression and prechordal plate development
Bessarab, D.A., Mathavan, S., Jones, C.M., Ray Dunn, N.
Date: 2015
Source: Differentiation; research in biological diversity   89(1-2): 22-30 (Journal)
Registered Authors: Bessarab, Dmitri, Mathavan, S.
Keywords: Alternative splicing, Epiboly, Evolutionary conservation, Prechordal plate, Zebrafish, rnf111
MeSH Terms:
  • Alternative Splicing/genetics
  • Animals
  • Gastrula/growth & development
  • Humans
  • Mice
  • Morphogenesis/genetics*
  • Nodal Signaling Ligands/genetics
  • Protein Isoforms/genetics*
  • Protein Isoforms/isolation & purification
  • Transcription, Genetic*
  • Ubiquitin-Protein Ligases/genetics
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed: 25619648 Full text @ Differentiation
Arkadia (also known as RING finger 111) encodes a nuclear E3 ubiquitin ligase that targets intracellular effectors and modulators of TGFβ/Nodal-related signaling for polyubiquitination and proteasome-dependent degradation. In the mouse, loss of Arkadia results in early embryonic lethality, with defects attributed to compromised Nodal signaling. Here, we report the isolation of zebrafish arkadia/rnf111, which is represented by 5 transcript variants. arkadia/rnf111 is broadly expressed during the blastula and gastrula stages, with eventual enrichment in the anterior mesendoderm, including the prechordal plate. Morpholino knockdown experiments reveal an unexpected role for Arkadia/Rnf111 in both early blastula organization and epiboly progression. Using a splice junction morpholino, we present additional evidence that arkadia/rnf111 transcript variants containing a 3' alternative exon are specifically required for epiboly progression in the late gastrula. This result suggests that arkadia/rnf111 transcript variants encode functionally relevant protein isoforms that provide additional intracellular flexibility and regulation to the Nodal signaling pathway.