PUBLICATION
Zebrafish Rnf111 is encoded by multiple transcripts and is required for epiboly progression and prechordal plate development
- Authors
- Bessarab, D.A., Mathavan, S., Jones, C.M., Ray Dunn, N.
- ID
- ZDB-PUB-150127-6
- Date
- 2015
- Source
- Differentiation; research in biological diversity 89(1-2): 22-30 (Journal)
- Registered Authors
- Bessarab, Dmitri, Mathavan, S.
- Keywords
- Alternative splicing, Epiboly, Evolutionary conservation, Prechordal plate, Zebrafish, rnf111
- MeSH Terms
-
- Humans
- Alternative Splicing/genetics
- Ubiquitin-Protein Ligases/genetics
- Morphogenesis/genetics*
- Zebrafish/genetics*
- Zebrafish/growth & development
- Mice
- Gastrula/growth & development
- Protein Isoforms/genetics*
- Protein Isoforms/isolation & purification
- Animals
- Nodal Signaling Ligands/genetics
- Transcription, Genetic*
- PubMed
- 25619648 Full text @ Differentiation
Citation
Bessarab, D.A., Mathavan, S., Jones, C.M., Ray Dunn, N. (2015) Zebrafish Rnf111 is encoded by multiple transcripts and is required for epiboly progression and prechordal plate development. Differentiation; research in biological diversity. 89(1-2):22-30.
Abstract
Arkadia (also known as RING finger 111) encodes a nuclear E3 ubiquitin ligase that targets intracellular effectors and modulators of TGFβ/Nodal-related signaling for polyubiquitination and proteasome-dependent degradation. In the mouse, loss of Arkadia results in early embryonic lethality, with defects attributed to compromised Nodal signaling. Here, we report the isolation of zebrafish arkadia/rnf111, which is represented by 5 transcript variants. arkadia/rnf111 is broadly expressed during the blastula and gastrula stages, with eventual enrichment in the anterior mesendoderm, including the prechordal plate. Morpholino knockdown experiments reveal an unexpected role for Arkadia/Rnf111 in both early blastula organization and epiboly progression. Using a splice junction morpholino, we present additional evidence that arkadia/rnf111 transcript variants containing a 3' alternative exon are specifically required for epiboly progression in the late gastrula. This result suggests that arkadia/rnf111 transcript variants encode functionally relevant protein isoforms that provide additional intracellular flexibility and regulation to the Nodal signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping