PUBLICATION
Characterization of the duplicate L-SIGN and DC-SIGN genes in miiuy croaker and evolutionary analysis of L-SIGN in fishes
- Authors
- Shu, C., Wang, S., Xu, T.
- ID
- ZDB-PUB-150118-1
- Date
- 2015
- Source
- Developmental and comparative immunology 50(1): 19-25 (Journal)
- Registered Authors
- Keywords
- DC-SIGN, L-SIGN, Miiuy croaker, evolutionary analysis, expression patterns, gene synteny
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Evolution, Molecular
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Fishes/genetics*
- Kidney/metabolism
- Liver/metabolism
- Molecular Sequence Data
- Perciformes/genetics*
- Phylogeny
- Spleen/metabolism
- PubMed
- 25596146 Full text @ Dev. Comp. Immunol.
Citation
Shu, C., Wang, S., Xu, T. (2015) Characterization of the duplicate L-SIGN and DC-SIGN genes in miiuy croaker and evolutionary analysis of L-SIGN in fishes. Developmental and comparative immunology. 50(1):19-25.
Abstract
Dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN/CD209) and liver/lymph node-specific ICAM-grabbing non-integrin (L-SIGN/CD299) which homologue of DC-SIGN are important members in C-type lectin receptors family as key molecules to recognize and eliminate pathogens in the innate immune system. DC-SIGN and L-SIGN have become hot topic in recent studies which both served as cell adhesion and phagocytic pathogen recognition receptors in mammals. However, there have been almost no studies of DC-SIGN and L-SIGN structure and characters in fish, only DC-SIGN in the zebrafish had been studied. In our study, we identified and characterized the full-length miiuy croaker (Miichthys miiuy) DC-SIGN (mmDC-SIGN) and L-SIGN (mmL-SIGN) genes. The sequence analysis results showed mmDC-SIGN and mmL-SIGN have the same domains with other vertebrates except primates and share some conserved motifs in CRD among all the vertebrates which play a crucial role in interacting with Ca(2+) and for recognizing mannose-containing motifs. Gene synteny of DC-SIGN and L-SIGN were analyzed for the first time and gene synteny of L-SIGN was conserved among the five fishes. Interestingly, one gene next to L-SIGN from gene synteny had high similarity with L-SIGN gene that was described as L-SIGN-like in fish species. While only one L-SIGN gene existed in other vertebrates, there are two L-SIGN in fish maybe in consequence of the fish-specific genome duplication to adapt the specific environment. The evolutionary analysis showed that the ancestral lineages of L-SIGN gene in fishes experienced purifying selection and the current lineages of L-SIGN gene in fishes underwent positive selection, indicating that the ancestral lineages and current lineages of L-SIGN gene in fishes underwent different evolutionary patterns. Both mmDC-SIGN and mmL-SIGN were expressed in all tested tissues and ubiquitously up-regulated in infected liver, spleen and kidney at different sampling time points indicated that the mmDC-SIGN and mmL-SIGN participated in the immune response to defense against bacteria infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping