PUBLICATION

Cdc42 Mediates Bmp-Induced Sprouting Angiogenesis through Fmnl3-Driven Assembly of Endothelial Filopodia in Zebrafish

Authors
Wakayama, Y., Fukuhara, S., Ando, K., Matsuda, M., Mochizuki, N.
ID
ZDB-PUB-150115-9
Date
2015
Source
Developmental Cell   32: 109-22 (Journal)
Registered Authors
Fukuhara, Shigetomo, Mochizuki, Naoki
Keywords
none
MeSH Terms
  • Actin Cytoskeleton
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism*
  • Endothelium, Vascular/cytology*
  • Endothelium, Vascular/metabolism
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Neovascularization, Physiologic*
  • Pseudopodia/physiology*
  • Signal Transduction
  • Veins/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • cdc42 GTP-Binding Protein/genetics
  • cdc42 GTP-Binding Protein/metabolism*
PubMed
25584797 Full text @ Dev. Cell
Abstract
During angiogenesis in vivo, endothelial cells (ECs) at the tips of vascular sprouts actively extend filopodia that are filled with bundles of linear actin filaments. To date, signaling pathways involved in the formation of endothelial filopodia have been studied using in-vitro-cultured ECs that behave differently from those in vivo. Herein, we have delineated a signaling pathway that governs the assembly of endothelial filopodia during angiogenic sprouting of the caudal vein plexus (CVP) in zebrafish. During CVP formation, bone morphogenetic protein induces the extension of endothelial filopodia and their migration via Arhgef9b-mediated activation of Cdc42. Active Cdc42 binds to and stimulates Formin-like 3, an actin-regulatory protein of the formin family, which, in turn, promotes the extension of endothelial filopodia to facilitate angiogenic sprouting of the CVP. Thus, this study has elucidated molecular mechanisms underlying the formation of endothelial filopodia and their role in angiogenesis in vivo.
Genes / Markers
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Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes