PUBLICATION
The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line
- Authors
- Hayashi, L., Sheth, M., Young, A., Kruger, M., Wayman, G.A., Coffin, A.B.
- ID
- ZDB-PUB-150104-2
- Date
- 2015
- Source
- Neurotoxicology 46: 125-36 (Journal)
- Registered Authors
- Coffin, Allison
- Keywords
- Auditory, Bisphenol-A, Hair cell, Hearing, Lateral line, Polychlorinated biphenyl
- MeSH Terms
-
- Benzhydryl Compounds/toxicity*
- Enzyme Inhibitors/pharmacology
- Zebrafish/growth & development
- Hair Cells, Vestibular/drug effects*
- Animals, Genetically Modified
- Cell Death/drug effects
- Environmental Pollutants/toxicity*
- Oxidative Stress/drug effects
- Time Factors
- Polychlorinated Biphenyls/toxicity*
- Lateral Line System/cytology*
- Lateral Line System/drug effects
- Animals
- Analysis of Variance
- Dose-Response Relationship, Drug
- Glutathione/metabolism
- Larva
- Phenols/toxicity*
- Regeneration/drug effects
- Transcription Factor Brn-3C/genetics
- Transcription Factor Brn-3C/metabolism
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- PubMed
- 25556122 Full text @ Neurotoxicology
- CTD
- 25556122
Citation
Hayashi, L., Sheth, M., Young, A., Kruger, M., Wayman, G.A., Coffin, A.B. (2015) The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line. Neurotoxicology. 46:125-36.
Abstract
Environmental toxicants such as bisphenol-A (BPA) and polychlorinated biphenyls (PCBs) are prevalent in our water supply, soil, and many food products and can profoundly affect the central nervous system. Both BPA and PCBs can disrupt endocrine signaling, which is important for auditory development and function, but the effect of these toxicants on the auditory periphery is not understood. In this study we investigated the effect of PCB-95 and BPA on lateral line development, function, and regeneration in larval zebrafish. The lateral line is a system of mechanosensory hair cells on the exterior of the fish that are homologous to the hair cells located in the mammalian inner ear. We found that PCB-95 had no effect on lateral line development or hair cell survival. BPA also did not affect lateral line development, but instead had a significant effect on both hair cell survival and regeneration. BPA-induced hair cell loss is both dose-and time-dependent, with concentrations of 1μM or higher killing lateral line hair cells during a 24 hr exposure period. Pharmacologic manipulation experiments suggest that BPA kills hair cells via activation of oxidative stress pathways, similar to prior reports of BPA toxicity in other tissues. We also observed that hair cells killed with neomycin, a known ototoxin, failed to regenerate normally when BPA was present, suggesting that BPA in aquatic environments could impede innate regenerative responses in fishes. Collectively, these data demonstrate that BPA can have detrimental effects on sensory systems, both in aquatic life and perhaps in terrestrial organisms, including humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping