PUBLICATION
Controlled Intracellular Self-Assembly and Disassembly of 19F Nanoparticles for MR Imaging of Caspase 3/7 in Zebrafish
- Authors
- Yuan, Y., Sun, H., Ge, S., Wang, M., Zhao, H., Wang, L., An, L., Zhang, J., Zhang, H., Hu, B., Wang, J., Liang, G.
- ID
- ZDB-PUB-141230-7
- Date
- 2015
- Source
- ACS nano 9(1): 761-8 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Caspase 3/metabolism*
- Caspase 7/metabolism*
- Fluorine-19 Magnetic Resonance Imaging/methods*
- Glutathione/metabolism
- Hep G2 Cells
- Humans
- Intracellular Space/metabolism*
- Models, Molecular
- Molecular Conformation
- Nanoparticles/chemistry*
- Nanotechnology/methods*
- Zebrafish*
- PubMed
- 25544315 Full text @ ACS Nano
Citation
Yuan, Y., Sun, H., Ge, S., Wang, M., Zhao, H., Wang, L., An, L., Zhang, J., Zhang, H., Hu, B., Wang, J., Liang, G. (2015) Controlled Intracellular Self-Assembly and Disassembly of 19F Nanoparticles for MR Imaging of Caspase 3/7 in Zebrafish. ACS nano. 9(1):761-8.
Abstract
Compared to (1)H MRI, (19)F MRI provides higher selectivity but lower sensitivity. Therefore, the need to inject high doses of (19)F probe to improve its sensitivity for in vivo diagnosis remains a challenge. A "smart" strategy is needed that could locally concentrate a low-dose (19)F probe while avoiding the fast transverse relaxation of the probes. Locally self-assembling and disassembling (19)F nanoparticles may be an optimal measure to achieve this goal. Herein, we report a dual-functional probe 1 for glutathione (GSH)-controlled self-assembly and subsequent caspase3/7 (Casp3/7)-controlled disassembly of formed nanoparticles (i.e., 1-NPs). Consecutive assembly and disassembly of 1-NPs translate to "off" and "on" (19)F magnetic resonance (MR) signal states, respectively. Employing this "smart" strategy, we successfully used 1 for the consecutive detection of GSH and Casp3/7 activitiy in vitro and in cells, and imaging Casp3/7 activitiy in cells and in zebrafish at low doses with a 14.1 Tesla magnetic field.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping