CNS Myelination Requires Cytoplasmic Dynein Function
- Yang, M.L., Shin, J., Kearns, C.A., Langworthy, M.M., Snell, H., Walker, M.B., Appel, B.
- Developmental Dynamics : an official publication of the American Association of Anatomists 244(2): 134-45 (Journal)
- Registered Authors
- Appel, Bruce, Kearns, Christina, Langworthy, Melissa, Shin, Jimann, Snell, Heather, Walker, Macie B.
- Dynein, axon, myelination, oligodendrocyte, zebrafish
- MeSH Terms
- Axonal Transport/physiology*
- Cytoplasmic Dyneins/genetics
- Cytoplasmic Dyneins/metabolism*
- Gene Expression Regulation, Developmental/physiology
- Myelin Sheath/genetics
- Myelin Sheath/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 25488883 Full text @ Dev. Dyn.
Yang, M.L., Shin, J., Kearns, C.A., Langworthy, M.M., Snell, H., Walker, M.B., Appel, B. (2015) CNS Myelination Requires Cytoplasmic Dynein Function. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(2):134-45.
Background: Cytoplasmic dynein provides the main motor force for minus-end-directed transport of cargo on microtubules. Within the vertebrate central nervous system (CNS), proliferation, neuronal migration and retrograde axon transport are among the cellular functions known to require dynein. Accordingly, mutations of DYNC1H1, which encodes the heavy chain subunit of cytoplasmic dynein, have been linked to developmental brain malformations and axonal pathologies. Oligodendrocytes, the myelinating glial cell type of the CNS, migrate from their origins to their target axons and subsequently extend multiple long processes that ensheath axons with specialized insulating membrane. These processes are filled with microtubules, which facilitate molecular transport of myelin components. However, whether oligodendrocytes require cytoplasmic dynein to ensheath axons with myelin is not known. Results: We identified a mutation of zebrafish dync1h1 in a forward genetic screen that caused a deficit of oligodendrocytes. Using in vivo imaging and gene expression analyses, we additionally found evidence that dync1h1 promotes axon ensheathment and myelin gene expression. Conclusions: In addition to its well known roles in axon transport and neuronal migration, cytoplasmic dynein contributes to neural development by promoting myelination. This article is protected by copyright. All rights reserved.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes