PUBLICATION
Sex specific response in cholesterol level in zebrafish (Danio rerio) after long-term exposure of difenoconazole
- Authors
- Mu, X., Wang, K., Chai, T., Zhu, L., Yang, Y., Zhang, J., Pang, S., Wang, C., Li, X.
- ID
- ZDB-PUB-141209-14
- Date
- 2015
- Source
- Environmental pollution (Barking, Essex : 1987) 197: 278-86 (Journal)
- Registered Authors
- Keywords
- Cholesterol, Difenoconazole, Sex differences, Zebrafish
- MeSH Terms
-
- Animals
- Cholesterol/metabolism
- Dioxolanes/toxicity*
- Female
- Fungicides, Industrial/metabolism
- Fungicides, Industrial/toxicity*
- Liver/metabolism
- Male
- Triazoles/toxicity*
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity
- Zebrafish/metabolism
- Zebrafish/physiology*
- PubMed
- 25483594 Full text @ Environ. Pollut.
Citation
Mu, X., Wang, K., Chai, T., Zhu, L., Yang, Y., Zhang, J., Pang, S., Wang, C., Li, X. (2015) Sex specific response in cholesterol level in zebrafish (Danio rerio) after long-term exposure of difenoconazole. Environmental pollution (Barking, Essex : 1987). 197:278-86.
Abstract
Difenoconazole is a widely used triazole fungicide, its extensive application may potentially cause toxic effects on non-target organisms. To investigate the effect of difenoconazole on cholesterol content and related mechanism, adult zebrafish were exposed to environmental related dosage (0.1, 10 and 500 μg/L) difenoconazole. The body weight and hepatic total cholesterol (TCHO) level was tested at 7, 15 and 30 days post exposure (dpe). The expressions of eight cholesterol synthesis genes and one cholesterol metabolism gene were assessed via Quantitative PCR method. The significant decrease of TCHO level in male zebrafish liver was observed at 15 and 30 dpe, which was accompanied by apparent hepatic cholesterol-genesis genes expression decline. In comparison with males, female zebrafish showed different transcription modification of tested genes, and the cholesterol content maintain normal level during the whole exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping