PUBLICATION
Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae
- Authors
- Zhu, B., Wang, Q., Wang, X., Zhou, B.
- ID
- ZDB-PUB-141203-34
- Date
- 2014
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 157C: 186-195 (Journal)
- Registered Authors
- Keywords
- BDE-209, Co-exposure, Pb, Synergistic effects, Thyroid disruption, Thyroid hormones
- MeSH Terms
-
- Animals
- Drug Synergism
- Gene Expression Regulation/drug effects
- Halogenated Diphenyl Ethers/metabolism
- Halogenated Diphenyl Ethers/toxicity*
- Larva/drug effects
- Lead/metabolism
- Lead/toxicity*
- Prealbumin/genetics
- Thyroid Gland/drug effects*
- Thyroid Hormones/metabolism
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/physiology*
- PubMed
- 25456233 Full text @ Aquat. Toxicol.
Citation
Zhu, B., Wang, Q., Wang, X., Zhou, B. (2014) Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands). 157C:186-195.
Abstract
Polybrominated diphenyl ethers (PBDEs) and metals are the main contaminants at waste electrical and electronic equipment ("e-waste") recycling sites. However, the potential environmental health effects of mixtures of PBDEs and metals are not known. We investigated co-exposure of lead (Pb) with decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae. Seven groups of embryos/larvae of zebrafish were treated with Pb (0, 2, 5, 10, 15, 20, and 30μg/L), six groups were exposed to BDE-209 (0, 50, 100, 200, 400, and 800μg/L), and nine groups of zebrafish larvae were treated with Pb and BDE-209 (5, 10, and 20μg/L Pb; 50, 100, and 200μg/L BDE-209). Embryos/larvae were exposed from 2h post-fertilization (hpf) until 144hpf, and thyroid hormone (TH) content measured. Pb exposure significantly decreased whole-body TH contents (triiodothyroxine (T3) and thyroxine (T4)) but BDE-209 exposure significantly increased T3 and T4 levels. Pb or BDE-209 treatment alone caused a predicted downregulation of TH transport (i.e., expression of the mRNA or proteins of transthyretin). Chemical analyses showed Pb uptake to be increased by BDE-209, but BDE-209 bioconcentration was decreased and the ability to metabolize BDE-209 was reduced in the presence of Pb. We also found that a mixture of the two chemicals had a synergistic effect on TH levels in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping