Hepatic positive and negative antioxidant responses in zebrafish after intraperitoneal administration of toxic microcystin-LR

Hou, J., Li, L., Xue, T., Long, M., Su, Y., Wu, N.
Chemosphere   120C: 729-736 (Journal)
Registered Authors
Li, Li
Antioxidant systems, Gene expression, Histopathology, Microcystin-LR, Zebrafish
MeSH Terms
  • Analysis of Variance
  • Animals
  • Antioxidants/metabolism*
  • Catalase/metabolism
  • Chromatography, High Pressure Liquid
  • DNA Primers/genetics
  • Dose-Response Relationship, Drug
  • Female
  • Gene Expression Regulation, Enzymologic/drug effects*
  • Glutathione/metabolism
  • Glutathione Peroxidase/metabolism
  • Glutathione Transferase/metabolism
  • Injections, Intraperitoneal
  • Liver/metabolism
  • Microcystins/administration & dosage
  • Microcystins/toxicity*
  • Oxidative Stress/drug effects*
  • Real-Time Polymerase Chain Reaction
  • Superoxide Dismutase/metabolism
  • Zebrafish/metabolism*
25462319 Full text @ Chemosphere
Microcystin-LR (MC-LR) is the most toxic and common among microcystins. In order to understand the possible molecular mechanisms of hepatic antioxidation and detoxification, the activities and transcriptional levels of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferases (GST), and glutathione (GSH) contents as well as histopathological changes were studied in the liver of female zebrafish injected intraperitoneally (i.p.) at doses of 50 and 200μg MC-LR kg(-1) body weight (BW) respectively. In the low dose group (50μg MC-LR kg(-1)), zebrafish displayed a little unease at the initial 1h post-injection (hpi), slight hepatic injury and quick recovery, and enhanced enzymatic activities and up-regulated gene expression of antioxidant enzymes. In contrast, high dose of MC-LR (200μg MC-LR kg(-1)) resulted in uneasiness and frantic swimming, severe hepatic injury, and suppressed enzymatic activities and down-regulated gene expression of antioxidant enzymes. GSH depletion in both dose groups may be explained by enhanced antioxidant reactions and higher rates of MC conjugation, suggesting the crucial roles of GSH in both cellular antioxidant protection and MC-LR detoxification. This study demonstrated that administration of MC-LR caused a positive response in the low dose group but a negative response in the high dose group. Hepatic positive/negative responses in the low/high dose group might result from an increased/decreased synthesis of antioxidant enzymes at the molecular level, respectively. These results illustrated that antioxidant status played an important role in zebrafish protection against MC-LR-caused oxidative stress through regulating antioxidant enzyme gene expression and activities.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes