PUBLICATION
Toxicogenomic analysis in the combined effect of tributyltin and benzo[a]pyrene on the development of zebrafish embryos
- Authors
- Huang, L., Zuo, Z., Zhang, Y., Wang, C.
- ID
- ZDB-PUB-141202-1
- Date
- 2015
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 158C: 157-164 (Journal)
- Registered Authors
- Keywords
- BaP, Combined effects, Microarray, TBT, Zebrafish
- MeSH Terms
-
- Animals
- Benzo(a)pyrene/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Oxidative Stress/drug effects
- Toxicogenetics
- Trialkyltin Compounds/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- PubMed
- 25438121 Full text @ Aquat. Toxicol.
Citation
Huang, L., Zuo, Z., Zhang, Y., Wang, C. (2015) Toxicogenomic analysis in the combined effect of tributyltin and benzo[a]pyrene on the development of zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 158C:157-164.
Abstract
There is a growing recognition that the toxic effects of chemical mixtures are been an important issue in toxicological sciences. Tributyltin (TBT) and benzo[a]pyrene (BaP) are widespread pollutants that occur simultaneously in the aquatic environments. This study was designed to examine comprehensively the combined effects of TBT and BaP on zebrafish (Danio rerio) embryos using toxicogenomic approach combined with biochemical detection and morphological analysis, and tried to gain insight into the mechanisms underlying the combined effects of TBT and BaP. The results of toxicogenomic data indicated that: (1) TBT cotreatment rescued the embryos from decreased hatching ratio caused by BaP alone, while the alteration of gene expression (in this article the phrase gene expression is used as a synonym to gene transcription, although in is acknowledged that gene expression can also be regulated by, e.g., translation and mRNA or protein stability) relative to zebrafish hatching in the BaP groups was resumed by the cotreatment with TBT; (2) BaP cotreatment decreased TBT-mediated dorsal curvature, and alleviated the perturbation of Notch pathway caused by TBT alone; (3) cotreatment with TBT decreased BaP-mediated bradycardia, which might be due to that TBT cotreatment alleviated the perturbation in expression of genes related to cardiac muscle cell development and calcium handling caused by BaP alone; 4) TBT cotreatment brought an antagonistic effect on the BaP-mediated oxidative stress and DNA damage. These results suggested that toxicogenomic approach was available for analyzing combined toxicity with high sensitivity and accuracy, which might improve our understanding and predictability for the combined effects of chemicals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping