ZFIN ID: ZDB-PUB-141120-4
The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish
Sander, V., Patke, S., Sahu, S., Teoh, C.L., Peng, Z., Chang, Y.T., Davidson, A.J.
Date: 2015
Source: Chemical communications (Cambridge, England)   51(2): 395-8 (Journal)
Registered Authors: Davidson, Alan
Keywords: none
MeSH Terms:
  • Animals
  • Fluorescent Dyes/administration & dosage
  • Fluorescent Dyes/analysis*
  • Kidney/anatomy & histology
  • Kidney/ultrastructure*
  • Kidney Tubules, Proximal/anatomy & histology
  • Kidney Tubules, Proximal/ultrastructure*
  • Larva/anatomy & histology
  • Larva/ultrastructure
  • Optical Imaging
  • Zebrafish/anatomy & histology*
  • Zebrafish/growth & development
PubMed: 25407666 Full text @ Chem. Commun. (Camb.)
ABSTRACT
We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function.
ADDITIONAL INFORMATION