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ZFIN ID: ZDB-PUB-141114-5
Blocking VEGF Signaling Delays Development of Replacement Teeth in Zebrafish
Crucke, J., Huysseune, A.
Date: 2015
Source: Journal of dental research   94(1): 157-65 (Journal)
Registered Authors: Huysseune, Ann
Keywords: Danio rerio, SU5416, blood supply, dentition, tooth replacement, vascular endothelial growth factor
MeSH Terms:
  • Alkaline Phosphatase/analysis
  • Angiogenesis Inhibitors/pharmacology
  • Animals
  • Blood Vessels/drug effects
  • Cell Differentiation/drug effects
  • Embryo, Nonmammalian/drug effects
  • Indoles/pharmacology
  • Larva/drug effects
  • Neovascularization, Physiologic/drug effects
  • Odontogenesis/drug effects*
  • Pyrroles/pharmacology
  • Tooth/blood supply
  • Tooth/drug effects
  • Tooth/growth & development*
  • Tooth Germ/drug effects
  • Vascular Endothelial Growth Factor A/antagonists & inhibitors*
  • Zebrafish/growth & development*
  • Zebrafish Proteins/antagonists & inhibitors*
PubMed: 25391620 Full text @ J. Dent. Res.
The dentition in zebrafish is extremely and richly vascularized, but the function of the vasculature, in view of the continuous replacement of the teeth, remains elusive. Through application of SU5416, a vascular endothelial growth factor receptor inhibitor, we studied the role of the blood vessels in the dentition of the zebrafish. We were unable to show an effect on the development of first-generation teeth as well as first tooth replacement. However, in juvenile fish, a delay was observed in the developmental state of the replacement tooth compared with what was expected based on the maturation state of the functional tooth. Furthermore, we observed a difference between treated and nontreated fish in the distance between blood vessels and developing replacement teeth. In conclusion, our results provide support for a nutritive, rather than an inductive, function of the vasculature in the process of tooth development and replacement.