PUBLICATION
Protective Role of Comfrey Leave Extracts on UV-induced Zebrafish Fin Damage
- Authors
- Cheng, C.C., Chou, C.Y., Chang, Y.C., Wang, H.W., Wen, C.C., Chen, Y.H.
- ID
- ZDB-PUB-141030-4
- Date
- 2014
- Source
- Journal of Toxicologic Pathology 27: 115-21 (Journal)
- Registered Authors
- Chen, Yau-Hung
- Keywords
- UV, comfrey, fin, zebrafish
- MeSH Terms
- none
- PubMed
- 25352712 Full text @ J. Toxicol. Pathol.
Citation
Cheng, C.C., Chou, C.Y., Chang, Y.C., Wang, H.W., Wen, C.C., Chen, Y.H. (2014) Protective Role of Comfrey Leave Extracts on UV-induced Zebrafish Fin Damage. Journal of Toxicologic Pathology. 27:115-21.
Abstract
In zebrafish, UV exposure leads to fin malformation phenotypes including fin reduction or absence. The present study evaluated UV-protective activities of comfrey leaves extracts in a zebrafish model by recording fin morphological changes. Chemopreventive effects of comfrey leave extracts were evaluated using Kaplan-Meier analysis and Cox proportional hazards regression. The results showed that (1) the mean times of return to normal fin in the UV+comfrey (50 and 100 ppm) groups were 3.43 and 2.86 days and were quicker compared with that in the UV only group (4.21 days); (2) zebrafish fins in the UV+comfrey (50 and 100 ppm) groups were 2.05 and 3.25 times more likely to return to normal than those in the UV only group; and (3) comfrey leave extracts had UV-absorbance abilities and significantly reduced ROS production in UV-exposed zebrafish embryos, which may attenuate UV-mediated apoptosis. In conclusion, comfrey leaves extracts may have the potential to be developed as UV-protective agents to protect zebrafish embryos from UV-induced damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping