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ZIRC
ZFIN ID: ZDB-PUB-141007-3
The epigenetic factor Kmt2a/Mll1 regulates neural progenitor proliferation and neuronal and glial differentiation
Huang, Y.C., Shih, H.Y., Lin, S.J., Chiu, C.C., Ma, T.L., Yeh, T.H., Cheng, Y.C.
Date: 2015
Source: Developmental Neurobiology   75(5): 452-62 (Journal)
Registered Authors:
Keywords: Kmt2a, differentiation, neural progenitors, proliferation, zebrafish
MeSH Terms:
  • Animals
  • Cell Differentiation/physiology*
  • Cell Proliferation*
  • Gene Expression Regulation, Developmental/physiology*
  • Histone-Lysine N-Methyltransferase/metabolism*
  • Neural Stem Cells/cytology*
  • Neurogenesis/physiology*
  • Neuroglia/cytology*
  • Neurons/physiology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed: 25284327 Full text @ Dev. Neurobiol.
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ABSTRACT
Multiple epigenetic factors play a critical role in cell proliferation and differentiation. However, their function in embryogenesis, especially in neural development, is currently unclear. The Trithorax group (TrxG) homolog KMT2A (MLL1) is an important epigenetic regulator during development and has an especially well-defined role in hematopoiesis. Translocation and aberrant expression of KMT2A is often observed in many tumors, indicating its proto-oncogenic character. Here, we show that Kmt2a was essential for neural development in zebrafish embryos. Disrupting the expression of Kmt2a using morpholino antisense oligonucleotides and a dominant-negative variant resulted in neurogenic phenotypes, including downregulated proliferation of neural progenitors, premature differentiation of neurons and impaired gliogenesis. This study therefore revealed a novel function of Kmt2a in cell proliferation and differentiation, providing further insight into the function of TrxG proteins in neural development and brain tumors.
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