ZFIN ID: ZDB-PUB-141005-5
ETV6 (TEL1) regulates embryonic hematopoiesis in zebrafish
Rasighaemi, P., Onnebo, S.M., Liongue, C., Ward, A.C.
Date: 2015
Source: Haematologica   100(1): 23-31 (Journal)
Registered Authors: Liongue, Clifford, Rasighaemi, Parisa, Ward, Alister, Ward, Alister C.
Keywords: ETV6, Granulocytes, Monocytes, Macrophages, Hematopoiesis, Hematopoietic Stem Cell, Red Cells
MeSH Terms:
  • Animals
  • Cell Differentiation*
  • Cell Lineage
  • Cells, Cultured
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Erythrocytes/cytology
  • Erythrocytes/metabolism
  • Gene Expression Regulation, Developmental*
  • Hematopoiesis/physiology*
  • Humans
  • Immunoenzyme Techniques
  • Macrophages/cytology
  • Macrophages/metabolism
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Morpholinos/pharmacology
  • Myeloid Cells/cytology
  • Myeloid Cells/metabolism
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 25281506 Full text @ Haematologica
Chromosomal translocations involving fusions of the human ETV6 (TEL1) gene occur frequently in hematological malignancies. However, a detailed understanding of the normal function of ETV6 remains incomplete. This study has employed zebrafish as a relevant model to investigate the role of ETV6 during embryonic hematopoiesis. Zebrafish possessed a single conserved etv6 orthologue that was expressed from 12 hpf in the lateral plate mesoderm, and later in hematopoietic, vascular and other tissues. Morpholino-mediated gene knockdown of etv6 revealed the complex contribution of this gene toward embryonic hematopoiesis. During primitive hematopoiesis, etv6 knockdown resulted in reduced levels of progenitor cells, erythrocyte and macrophage populations, but increased numbers of incompletely differentiated heterophils. Definitive hematopoiesis was also perturbed, with etv6 knockdown leading to decreased erythrocytes and myeloid cells, but enhanced lymphopoiesis. This study suggests that ETV6 plays a broader and more complex role in early hematopoiesis than previously thought, impacting on the development of multiple lineages.