PUBLICATION
Progesterone and norgestrel alter transcriptional expression of genes along the hypothalamic-pituitary-thyroid axis in zebrafish embryos-larvae
- Authors
- Liang, Y.Q., Huang, G.Y., Ying, G.G., Liu, S.S., Jiang, Y.X., Liu, S.
- ID
- ZDB-PUB-141004-5
- Date
- 2015
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 167: 101-7 (Journal)
- Registered Authors
- Keywords
- Gene transcription, Hypothalamic-pituitary-thyroid axis, Norgestrel, Progesterone
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects*
- Gene Expression Regulation, Developmental/drug effects*
- Hypothalamo-Hypophyseal System/drug effects*
- Larva/drug effects
- Norgestrel/pharmacology*
- Progesterone/pharmacology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Thyroid Gland/drug effects
- Transcription, Genetic
- Zebrafish/embryology*
- PubMed
- 25277675 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Liang, Y.Q., Huang, G.Y., Ying, G.G., Liu, S.S., Jiang, Y.X., Liu, S. (2015) Progesterone and norgestrel alter transcriptional expression of genes along the hypothalamic-pituitary-thyroid axis in zebrafish embryos-larvae. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 167:101-7.
Abstract
The aim of this study was to investigate the effects of progestins on the hypothalamic-pituitary-thyroid (HPT) axis in the early stage of zebrafish. Zebrafish embryos were exposed to progesterone (P4) or norgestrel (NGT) at 5, 50 and 100ngL(-1) for 144h post fertilization (hpf), and the transcriptional levels of target genes along the hypothalamic-pituitary-thyroid axis were determined daily. The results showed that P4 had only minor effects on the mRNA expression of thyroglobulin (Tg), iodothyronine deiodinase type Ι (Dio1) and thyroid hormone receptor β (Thrb) genes. Similarly, the effects of NGT on transcripts of thyrotropin-releasing hormone (Trh), Dio1, iodothyronine deiodinase type II (Dio2) and thyroid hormone receptor α (Thra) genes were generally low. In addition, NGT resulted in some alterations of Tg and Thrb transcripts at different time points. However, a strong induction of Nis mRNA by P4 and NGT was observed in zebrafish embryos-larvae. The overall results showed that besides Nis no effects on the hypothalamic-pituitary-thyroid (HPT) axis are observed following exposure to P4 and NGT, which imply that both P4 and NGT have potential effects on the thyroid endocrine system by inducing transcript of Nis gene during the early stage of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping