PUBLICATION
Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus
- Authors
- Cai, W.J., Yuan, X.C., Yuan, Y.C., Xie, S.Q., Gong, Y., Su, H., Qiao, Y.
- ID
- ZDB-PUB-140923-4
- Date
- 2015
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 179: 54-61 (Journal)
- Registered Authors
- Keywords
- GH, GHS-R, Grass carp, cDNA cloning, energy homeostasis, ghrelin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps/blood
- Carps/genetics*
- Cloning, Molecular
- Fasting
- Food Deprivation
- Gene Expression Profiling
- Gene Expression Regulation*
- Genome*
- Ghrelin/administration & dosage
- Ghrelin/genetics
- Ghrelin/metabolism
- Growth Hormone/blood
- Molecular Sequence Data
- Organ Specificity/genetics
- Phylogeny
- Pituitary Gland/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Receptors, Ghrelin/chemistry
- Receptors, Ghrelin/genetics*
- Receptors, Ghrelin/metabolism
- Sequence Alignment
- Sequence Analysis, DNA*
- PubMed
- 25242546 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Cai, W.J., Yuan, X.C., Yuan, Y.C., Xie, S.Q., Gong, Y., Su, H., Qiao, Y. (2015) Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 179:54-61.
Abstract
The growth hormone secretagogue-receptor (GHS-R) is an endogenous receptor for the gut hormone ghrelin. Here we report the identification and characterization of GHS-R1a in grass carp, Ctenopharyngodon idellus. The full-length GHS-R1a cDNA contained a 1803-bp coding domain sequence which encoded a peptide of 360 amino acid residues. Comparison analysis revealed that the amino acid sequences of GHS-R1a were highly conserved in vertebrates shared 97% amino acid identity with zebrafish (Danio rerio), 96% with jian carp (Cyprinus carpio var. Jian) and 93% with goldfish (Carassius auratus). The GHS-R1a showed the highest level of mRNA expression in the pituitary, followed by brain, liver, and lowest expression was observed in hindgut. Intraperitoneally injected with grass carp ghrelin (50, 100 and 150ng/g body weight (BW)), grass carp showed greater mRNA expression of GHS-R1a in the pituitary compared with saline injected at 0.5h post injection. It was observed that food deprivation could promote the expression of ghrelin and GHS-R1a in the pituitary, demonstrating that nutritional status can influence the expression of both ghrelin and GHS-R1a in the pituitary. After 2- or 4-week fast, plasma growth hormone (GH) increased, were positively correlated with ghrelin and GHS-R1a mRNA expression levels in the pituitary. These results suggested that the involvement of ghrelin/GHS-R1a systems in mediating the effects of nutritional status and ghrelin in grass carp.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping