PUBLICATION
BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies
- Authors
- Kraus, P., Winata, C.L., Lufkin, T.
- ID
- ZDB-PUB-140923-10
- Date
- 2015
- Source
- Methods in molecular biology (Clifton, N.J.) 1227: 245-58 (Chapter)
- Registered Authors
- Winata, Cecilia Lanny
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified*
- Chromosomes, Artificial, Bacterial/chemistry
- Chromosomes, Artificial, Bacterial/genetics*
- Embryo, Nonmammalian
- Enhancer Elements, Genetic*
- Female
- Gene Expression Regulation, Developmental
- Gene Library
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Lac Operon
- Male
- Promoter Regions, Genetic*
- Transcription, Genetic
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zygote
- PubMed
- 25239750 Full text @ Meth. Mol. Biol.
Citation
Kraus, P., Winata, C.L., Lufkin, T. (2015) BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies. Methods in molecular biology (Clifton, N.J.). 1227:245-58.
Abstract
With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill demand and a more cost-effective technique. Here we provide guidelines for the generation of BAC recombineering-based transgenic zebrafish for DNA transcriptional promoter and enhancer identification studies as well as protocols for their analysis, which have been successfully applied in our laboratories many times. BAC recombineering in zebrafish allows for economical functional genomics studies, for example by integrating developmental biology with comparative genomics approaches to validate potential enhancer elements of vertebrate transcription factors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping