PUBLICATION
Anti-oxidant and anti-angiogenic properties of phenolic extract from Pleurotus tuber-regium
- Authors
- Lin, S., Lai, T.C., Chen, L., Kwok, H.F., Lau, C.B., Cheung, P.C.
- ID
- ZDB-PUB-140905-11
- Date
- 2014
- Source
- Journal of Agricultural and Food Chemistry 62(39): 9488-98 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Antioxidants/chemistry
- Antioxidants/isolation & purification
- Antioxidants/pharmacology*
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/metabolism
- Human Umbilical Vein Endothelial Cells/cytology
- Human Umbilical Vein Endothelial Cells/drug effects
- Humans
- Matrix Metalloproteinases/genetics
- Matrix Metalloproteinases/metabolism
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/genetics
- Neovascularization, Pathologic/metabolism
- Plant Extracts/chemistry
- Plant Extracts/isolation & purification
- Plant Extracts/pharmacology*
- Pleurotus/chemistry*
- Vegetables/chemistry*
- Zebrafish
- PubMed
- 25185869 Full text @ J. Agric. Food Chem.
Citation
Lin, S., Lai, T.C., Chen, L., Kwok, H.F., Lau, C.B., Cheung, P.C. (2014) Anti-oxidant and anti-angiogenic properties of phenolic extract from Pleurotus tuber-regium. Journal of Agricultural and Food Chemistry. 62(39):9488-98.
Abstract
Pleurotus tuber-regium (Fries) Singer (PTR), both an edible and medicinal mushroom also known as tiger milk mushroom, has a growing popularity and economic importance due to its flavor, nutritive value and medicinal effects. In this study, the anti-oxidant and anti-angiogenic activities of a 60% ethanol extract (EE) obtained from the sclerotium of PTR was investigated. Typical phenolic compounds including protocatechuic, chlorogenic, syringic, ferulic, folic acid were identified and quantified in EE by the HPLC-UV-ESI/MS analyses. EE possessed strong antioxidant activity and could dose-dependently inhibit vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cells (HUVEC) migration and tube formation. qPCR results showed that VEGF-induced FGF, ANG-TIE and MMP gene expression as well as VEGFR were down-regulated at the mRNA level after treated with EE, suggesting that multiple molecular targets related to angiogenesis was involved. Furthermore, EE also inhibited the formation of sub-intestinal vessel plexus (SIVs) in zebrafish embryos in vivo. All of these suggested that EE of PTR could be the source of potential inhibitors to target angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping